CAJ | 학술논문

用20亿PIB/mL灰茶尺蠖病毒(EgNPV)对成年SD大鼠进行急性经口、经皮毒性试验,对白色家兔进行眼刺激和皮肤刺激试验,对豚鼠进行皮肤致敏试验以及进行小鼠急性致病性试验.结果表明20亿PIB/mL灰茶尺蠖病毒(EgNPV)水剂对SD大鼠的急性经口毒性(其LD_(50)雌雄大鼠均>5 000 mg/kg)属于低毒;对SD大鼠的急性经皮毒性(其LD_(50)雌雄大鼠均>2 000 mg/kg)属于低毒;对家兔的皮肤无刺激性;对家兔的眼睛也无刺激性:在染毒后24 h内不洗眼情况下,眼刺激积分最高为0;对豚鼠的皮肤致敏率为0,其致敏性分级为Ⅰ级,属弱致敏物;病理组织学检查结果表明,各实验组小鼠内脏组织均未见有病理改变,无急性致病性.
용20억PIB/mL회다척확병독(EgNPV)대성년SD대서진행급성경구、경피독성시험,대백색가토진행안자격화피부자격시험,대돈서진행피부치민시험이급진행소서급성치병성시험.결과표명20억PIB/mL회다척확병독(EgNPV)수제대SD대서적급성경구독성(기LD_(50)자웅대서균>5 000 mg/kg)속우저독;대SD대서적급성경피독성(기LD_(50)자웅대서균>2 000 mg/kg)속우저독;대가토적피부무자격성;대가토적안정야무자격성:재염독후24 h내불세안정황하,안자격적분최고위0;대돈서적피부치민솔위0,기치민성분급위Ⅰ급,속약치민물;병리조직학검사결과표명,각실험조소서내장조직균미견유병리개변,무급성치병성.
A series of toxicological researches conducted in this study. the acute toxicity test, the eyes and skin stimulative test on rabbit, the skin sensitivity test on Guinea pig and the acute pathogenic test on mice. According to the experimental results of acute oral toxicity (LD_(50)>5 000 mg/kg) and acute dermal toxicity(LD_(50)>2 000 mg/kg), the acute toxicity of EgNPV on mice belongs to the category of low toxicity. It is not only non-irritating to skin but also has no stimulation on eyes of rabbits. The sensitization rate was 0%, did not cause hypersensitic reaction on Guinea pig. The results of histopathological test showed that no pathological changes were found in internal organs of mice in each experimental groups.