中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2010年
5期
431-437
,共7页
阿尔茨海默病%β淀粉样肽%脑灵汤%淀粉样前体蛋白%大鼠
阿爾茨海默病%β澱粉樣肽%腦靈湯%澱粉樣前體蛋白%大鼠
아이자해묵병%β정분양태%뇌령탕%정분양전체단백%대서
Alzheimer's disease%β-amyloid polypeptide%Naoling decoction%APP%rat
目的:探讨脑灵汤对阿尔茨海默病(AD)模型大鼠海马CA3区APP蛋白表达的影响,并揭示AD发病的部分机制和脑灵汤可能的作用机制.方法:选取40只SPF清洁级SD大鼠,随机分为正常对照组、假手术组、AD组、脑灵汤组和脑复康组,每组8只,喂养1周后,采用Aβ1-42注射大鼠海马法制成大鼠模型,再喂养28 d,治疗后用Morris水迷宫实验检测大鼠学习记忆行为能力, HE染色检测大鼠海马组织形态学变化,免疫组织化学法观察APP蛋白在大鼠海马CA3区中的表达.结果:Morris水迷宫实验结显示:AD大鼠组的隐匿平台逃避潜伏期延长(P＜0.05),在探索实验中,AD大鼠存在记忆障碍(P＜0.05),而脑灵汤治疗后可使AD大鼠的逃避潜伏期缩短(P＜0.05),大鼠平均探索次数明显增多(P＜0.05).AD组大鼠海马CA3区锥体细胞排列紊乱,有细胞缺失的现象,APP蛋白表达增多,脑灵汤治疗后可改善CA3区椎体细胞的病理改变以及降低APP蛋白的表达.结论:脑灵汤能明显改善AD模型大鼠低下的空间学习记忆能力,降低模型大鼠海马CA3区APP表达,对老年性痴呆大鼠学习记忆能力可能有提高作用.
目的:探討腦靈湯對阿爾茨海默病(AD)模型大鼠海馬CA3區APP蛋白錶達的影響,併揭示AD髮病的部分機製和腦靈湯可能的作用機製.方法:選取40隻SPF清潔級SD大鼠,隨機分為正常對照組、假手術組、AD組、腦靈湯組和腦複康組,每組8隻,餵養1週後,採用Aβ1-42註射大鼠海馬法製成大鼠模型,再餵養28 d,治療後用Morris水迷宮實驗檢測大鼠學習記憶行為能力, HE染色檢測大鼠海馬組織形態學變化,免疫組織化學法觀察APP蛋白在大鼠海馬CA3區中的錶達.結果:Morris水迷宮實驗結顯示:AD大鼠組的隱匿平檯逃避潛伏期延長(P＜0.05),在探索實驗中,AD大鼠存在記憶障礙(P＜0.05),而腦靈湯治療後可使AD大鼠的逃避潛伏期縮短(P＜0.05),大鼠平均探索次數明顯增多(P＜0.05).AD組大鼠海馬CA3區錐體細胞排列紊亂,有細胞缺失的現象,APP蛋白錶達增多,腦靈湯治療後可改善CA3區椎體細胞的病理改變以及降低APP蛋白的錶達.結論:腦靈湯能明顯改善AD模型大鼠低下的空間學習記憶能力,降低模型大鼠海馬CA3區APP錶達,對老年性癡呆大鼠學習記憶能力可能有提高作用.
목적:탐토뇌령탕대아이자해묵병(AD)모형대서해마CA3구APP단백표체적영향,병게시AD발병적부분궤제화뇌령탕가능적작용궤제.방법:선취40지SPF청길급SD대서,수궤분위정상대조조、가수술조、AD조、뇌령탕조화뇌복강조,매조8지,위양1주후,채용Aβ1-42주사대서해마법제성대서모형,재위양28 d,치료후용Morris수미궁실험검측대서학습기억행위능력, HE염색검측대서해마조직형태학변화,면역조직화학법관찰APP단백재대서해마CA3구중적표체.결과:Morris수미궁실험결현시:AD대서조적은닉평태도피잠복기연장(P＜0.05),재탐색실험중,AD대서존재기억장애(P＜0.05),이뇌령탕치료후가사AD대서적도피잠복기축단(P＜0.05),대서평균탐색차수명현증다(P＜0.05).AD조대서해마CA3구추체세포배렬문란,유세포결실적현상,APP단백표체증다,뇌령탕치료후가개선CA3구추체세포적병리개변이급강저APP단백적표체.결론:뇌령탕능명현개선AD모형대서저하적공간학습기억능력,강저모형대서해마CA3구APP표체,대노년성치태대서학습기억능력가능유제고작용.
Objective To observe the effect of Naoling decoction on hippocamal histomorphology and the expression of amyloid precursor protein (APP) in CA3 region in rats with Alzheimer's disease (AD), and to explore the therapeutical and the potential mechanism. Methods Forty SD rats were classified into 5 groups: a normal group, a sham-operated group, an AD group, a Naoling decoction group, and a Naofukang group. Alzheimer's disease model was established by Aβ1-42 injected into the hippocamal in the rats. The faculty of learning and memory was evaluated by Morris water maze. The changes of cell morphology were detected by HE staining. Expression of APP in CA3 region was mea-sured with immunohistochemical staining. Results Morris water maze experiment showed that the escape latency of hidden platform in the AD group were delayed significantly (P＜0.05) and the average times of passing was decreased (P＜0.05). In the hippocampal CA3 field of the AD rats, HE staining and immunohistochemical test showed that pyramidal cells disturbed, neurons decreased significantly and expression of APP protein increased (P＜0.05). Naoling decoction treatment improved pyramidal cellular disorders and decreased APP expression. Conclusion Naoling decoction can distinctly improve the learning and memory ability, and reduce the expression of APP in the AD model rats, suggesting that Naoling decoction can be used for the treatment of AD in rats.
