CAJ | 학술논문

目的利用基因共表达meta分析3种药物成瘾机制.方法基于酒精、可卡因和海洛因在内的药物成瘾组及对照组的基因表达谱数据,采用基因共表达meta分析方法,筛选出显著的基因共表达对,构建两个共表达网络；结合通路信息和基因功能注释信息,识别随着基因共表达模式的变化而发生改变的功能和通路关联对.结果研究表明,嘌呤核苷酸代谢过程、心率调节、神经元突触适应性的长期调节、多细胞生物应激反应、联想性学习、cAMP代谢、γ-氨基丁酸信号通路、多巴胺受体信号通路、cdk5在阿尔茨海默症中失常的通路等均在成瘾过程中扮演重要角色.结论基因共表达meta分析可有效挖掘在成瘾条件下发生显著改变的生物学功能及通路,为阐述药物成瘾共同的机制提供帮助.
목적 이용기인공표체meta분석3충약물성은궤제.방법 기우주정、가잡인화해락인재내적약물성은조급대조조적기인표체보수거,채용기인공표체meta분석방법,사선출현저적기인공표체대,구건량개공표체망락；결합통로신식화기인공능주석신식,식별수착기인공표체모식적변화이발생개변적공능화통로관련대.결과 연구표명,표령핵감산대사과정、심솔조절、신경원돌촉괄응성적장기조절、다세포생물응격반응、련상성학습、cAMP대사、γ-안기정산신호통로、다파알수체신호통로、cdk5재아이자해묵증중실상적통로등균재성은과정중분연중요각색.결론 기인공표체meta분석가유효알굴재성은조건하발생현저개변적생물학공능급통로,위천술약물성은공동적궤제제공방조.
Objectives To detect the common mechanism shared by 3 drugs underlying addiction,using gene co-expression meta-analysis.Methods By applying co-expression meta-analysis method to mRNA expression profiles between normal samples and the samples related to alcohol,cocaine,and heroine,significant gene co-expression pairs were identified.As co-expression networks of drug group and control group were constructed,associated function term pairs and pathway pairs reflected by co-expression pattern changes were identified by integrating functional and pathway information respectively.Results The results indicated that purine nucleotide catabolic process,regulation of heart rate,regulation of longterm neuronal synaptic plasticity,multi-cellular organismal response to stress,associative learning,cAMP metabolic process,gamma-aminobutyric acid signaling pathway,dopamine receptor pathway,deregulation of cdk 5 in Alzheimers disease pathway may play an important role in drug addiction.Conclusion Gene co-expression meta-analysis can effectively identify the functions and pathways significantly changed in drug addiction.More importantly,we provided theory that will support the researches of addiction common mechanisms.