CAJ | 학술논문

目的探讨国产6711型125I粒子的相对生物效应,并对其照射杀伤PANC-1细胞的效果进行了初步探讨.方法 PANC-1细胞处于指数生长期时,行125I粒子离体照射;在初始剂量率为2.59 cGy/h时,分别给予1、2、4、6、8和10 Gy照射.60Co照射作为对照组,吸收剂量率为2.21 Gy/min,给予相同剂量照射.用锥虫蓝染色法检测细胞死亡比率,并比较在4 Gy照射后培养12、24、48和72 h细胞死亡率随时间变化.采用克隆形成实验,计算细胞克隆形成率,绘制生存曲线,得出生物学参数,并测定125I粒子与60Co的相对生物效应.结果在相同剂量照射下,125I持续低剂量率照射与60Co照射相比,当≥4 Gy时,细胞死亡率明显增高.在4 Gy照射后,随着时间延长细胞死亡率增高,两者相比有明显差异.从生存曲线分析,125I粒子持续低剂量率照射细胞存活分数比60Co低,125I粒子相对于60Co的生物效应为1.45.结论 125I粒子持续低剂量率照射与60Co高剂量率照射相比,细胞杀伤效应更强;测定的相对生物效应与其他研究者测量的结果相似;将为临床125I粒子治疗肿瘤提供一定的参考价值.
목적 탐토국산6711형125I입자적상대생물효응,병대기조사살상PANC-1세포적효과진행료초보탐토.방법 PANC-1세포처우지수생장기시,행125I입자리체조사;재초시제량솔위2.59 cGy/h시,분별급여1、2、4、6、8화10 Gy조사.60Co조사작위대조조,흡수제량솔위2.21 Gy/min,급여상동제량조사.용추충람염색법검측세포사망비솔,병비교재4 Gy조사후배양12、24、48화72 h세포사망솔수시간변화.채용극륭형성실험,계산세포극륭형성솔,회제생존곡선,득출생물학삼수,병측정125I입자여60Co적상대생물효응.결과 재상동제량조사하,125I지속저제량솔조사여60Co조사상비,당≥4 Gy시,세포사망솔명현증고.재4 Gy조사후,수착시간연장세포사망솔증고,량자상비유명현차이.종생존곡선분석,125I입자지속저제량솔조사세포존활분수비60Co저,125I입자상대우60Co적생물효응위1.45.결론 125I입자지속저제량솔조사여60Co고제량솔조사상비,세포살상효응경강;측정적상대생물효응여기타연구자측량적결과상사;장위림상125I입자치료종류제공일정적삼고개치.
Objective To investigate the relative biological effectiveness(RBE) of National Model 6711 125I seeds and the response patterns of PANC-1 exposed to 125I seeds irradiation.Methods PANC-1 cells inexponential growth were irradiated at initial dose rate of 2.59 cGy/h in vitro and exposed to l,2,4,6,8 and 10 Gy.Meanwhile,the other part of cells were exposed to the same doses by 60Co at dose rate of 2.21 Gy/min. After irradiation,the cells were stained by trypan blue to measure the cellular mortality rate and to compare the changes along with plating times of 12,24,48 and 72 h after 4 Gy.The colonies were counted to obtain the plating efficiencies by colony-forming assay and the cell surviving fraction was calculated tO plot cell survival curves,and RBE of 125I seeds relative to 60Co was determined.Results The cell death rate for continuous low- dose-rate(LDR)irradiation by 125I seeds was greater than 60Co at the same doses above or equal to 4 Gy.After 4 Gy irradiation,the cellular mortality rates were increased with times.The difference was significant between125 I seeds and 60Co.The survival fractions of 125I were lower than those of 60Co.and the RBE of 125I relative to 60Co was determined to be 1.45.Conclusion The cell-killing effects for continuous low-dose-rate(LDR)irradiation by 125I seeds are greater than acute high-dose.rate of 60Co.