CAJ | 학술논문

目的评价细胞间黏附分子-1单克隆抗体(1A29)对全脑缺血再灌注损伤大鼠的脑保护作用.方法健康成年雄性SD大鼠40只,体重180～200 g,2～3月龄,采用四血管法制备全脑缺血再灌注模型.随机分为4组(n=10):Ⅰ组于缺血前即刻股静脉注射同型对照抗体1 mg/kg;Ⅱ组～Ⅳ组分别于缺血前即刻、再灌注即刻及再灌注4 h时股静脉注射1A29 1 mg/kg.于再灌注24 h时进行神经功能损伤评分、脑组织多形核白细胞及单核细胞计数,并计算脑梗死面积百分比及脑组织含水量.结果与Ⅰ组比较,其余3组脑组织多形核白细胞计数、单核细胞计数、脑梗死面积百分比、含水量及神经功能损伤评分均明显降低(P<0.01),3组间差异无统计学意义(P>0.05).结论缺血前或再灌注4 h内静脉注射1A29均可减轻大鼠全脑缺血再灌注损伤.
목적 평개세포간점부분자-1단극륭항체(1A29)대전뇌결혈재관주손상대서적뇌보호작용.방법 건강성년웅성SD대서40지,체중180～200 g,2～3월령,채용사혈관법제비전뇌결혈재관주모형.수궤분위4조(n=10):Ⅰ조우결혈전즉각고정맥주사동형대조항체1 mg/kg;Ⅱ조～Ⅳ조분별우결혈전즉각、재관주즉각급재관주4 h시고정맥주사1A29 1 mg/kg.우재관주24 h시진행신경공능손상평분、뇌조직다형핵백세포급단핵세포계수,병계산뇌경사면적백분비급뇌조직함수량.결과 여Ⅰ조비교,기여3조뇌조직다형핵백세포계수、단핵세포계수、뇌경사면적백분비、함수량급신경공능손상평분균명현강저(P<0.01),3조간차이무통계학의의(P>0.05).결론 결혈전혹재관주4 h내정맥주사1A29균가감경대서전뇌결혈재관주손상.
Objective To investigate the cerebral protective effect of anti-intercellular adhesion molecule-1 (anti-ICAM-1) antibody (1A29) in rats with global cerebral ischemia/reperfusion (I/R) injury. Methods Forty healthy SD rats weighing 180-200 g were randomly divided into 4 groups ( n = 10 each) : group Ⅰ received isotype-matched control antibody 1 mg/kg iv (control group); group Ⅰ ,Ⅲ ,Ⅳ received 1A29 1 mg/kg iv before cerebral ischemia (group Ⅱ), at the onset of reperfusion (group Ⅲ) and at 4 h of reperfusion (group Ⅳ) respectively. The global cerebral I/R was produced by 30 min four-vessel occlusion followed by 24 h reperfusion. All animals were killed at 24 h of reperfusion. Their brains were removed for microscopic examination and measurement of W/D weight ratio, infarct size and neurological deficits (0 = no deficit,4 = unable to crawl with mental depression) .Results The PMN leukocyte and monocyte infiltration, water content of the brain and infarct size were significantly decreased in group Ⅱ ,Ⅲ and Ⅳ as compared with control group. The neurological deficit scores were significantly lower in group Ⅱ ,Ⅲ, Ⅳ(1.6-1.8) than in control group (2.5).Conclusion 1A29 antibody administered either before ischemia or during the 4 h of reperfusion can protect the brain against global cerebral I/R injury.