CAJ | 학술논문

目的探讨食管癌发生、发展过程中凋亡抑制蛋白Livin的表达及其与食管癌临床病理特征的关系.方法采用实时定量PCR法及蛋白免疫印迹法检测Livin在正常食管黏膜(10例)、不典型增生(10例)、原位癌(10例)及浸润癌(10例)组织中mRNA及蛋白的表达强度.结果 Livin mRNA在正常食管黏膜、不典型增生、原位癌及浸润癌组织中呈渐进性高表达[分别为1.00±0.00、2.26±0.79、7.24±1.06、12.21±2.47],原位癌、浸润癌与正常食管黏膜组织比较,差异有统计学意义(P均<0.05).结论 Livin与食管癌的发生、发展密切相关,有望成为食管癌诊断和基因治疗的新靶点.
목적 탐토식관암발생、발전과정중조망억제단백Livin적표체급기여식관암림상병리특정적관계.방법 채용실시정량PCR법급단백면역인적법검측Livin재정상식관점막(10례)、불전형증생(10례)、원위암(10례)급침윤암(10례)조직중mRNA급단백적표체강도.결과 Livin mRNA재정상식관점막、불전형증생、원위암급침윤암조직중정점진성고표체[분별위1.00±0.00、2.26±0.79、7.24±1.06、12.21±2.47],원위암、침윤암여정상식관점막조직비교,차이유통계학의의(P균<0.05).결론 Livin여식관암적발생、발전밀절상관,유망성위식관암진단화기인치료적신파점.
Objective To detect the expression of apoptotic inhibitor protein Livin in different esophageal epithelial lesions and to analyze the relation between the expression of Livin with pathologic characteristics. Methods The expressions of Livin mRNA and Livin protein were detected by real- time fluorescence quantitative PCR and western blot in 40 patients with different esophageal epithelial lesions including normal, atypical hyperplasia, carcino-ma in stiu and invasive carcinoma. Results The expressions of Livin mRNA were progressively increased from nor-mal to invasive carcinoma( 1.00 ± 0. 00,2.26 ± 0.79,7.24 ± 1.06,12.21 ± 2.47 ). There was statistical signifi-cance in Livin mRNA expression among carcinoma in stiu and invasive carcinoma and normal tissues ( P < 0.05 ). Conclusion The expression of Livin is significantly related to the progression of esophageal cancer,which may be a new target for diagnosis and gene treatment of esophageal carcinoma.