中国医师杂志
中國醫師雜誌
중국의사잡지
JOURNAL OF CHINESE PHYSICIAN
2009年
4期
488-490
,共3页
巨噬细胞游走抑制因子/生物合成%结肠炎%三硝基苯磺酸/副作用
巨噬細胞遊走抑製因子/生物閤成%結腸炎%三硝基苯磺痠/副作用
거서세포유주억제인자/생물합성%결장염%삼초기분광산/부작용
Macrophage migration-inhibitory factors/BI%Colitis%Trinitrobenzensulfonic acid/AE
目的 观察巨噬细胞移动抑制因子(MIF)在三硝基苯磺酸(TNBS)诱发的大鼠结肠炎肠组织中的表达及其与疾病严重程度的关系.方法 采用TNBS/乙醇溶液灌肠制备大鼠结肠炎模型,24只动物分为正常组、轻、重模型组共3组.每天观察各组大鼠疾病活动指数(DAI);生化法检测髓过氧化物酶(MPO)活性,采用逆转录-聚合酶链反应(RT-PCR)技术,对肠组织MIF的表达水平进行半定量测定.结果 正常组DAI、MPO活性及MIFmRNA的表达水平分别为0.51±0.28、0.38±0.18、0.11±0.03;模型Ⅰ组5.04±0.73、1.68±0.45、0.65±0.04;模型Ⅱ组8.13±0.71、2.70±0.35、0.81±0.05.与正常组相比,模型组肠组织中MIFmR-NA的表达显著增强(P<0.05),MPO活性及DAI也显著增高(P<0.05),且在病情严重组增高尤为明显.结论 大鼠结肠炎肠组织中MIF的表达升高,并与疾病严重程度正相关,提示其可能与大鼠结肠炎的发病有关.
目的 觀察巨噬細胞移動抑製因子(MIF)在三硝基苯磺痠(TNBS)誘髮的大鼠結腸炎腸組織中的錶達及其與疾病嚴重程度的關繫.方法 採用TNBS/乙醇溶液灌腸製備大鼠結腸炎模型,24隻動物分為正常組、輕、重模型組共3組.每天觀察各組大鼠疾病活動指數(DAI);生化法檢測髓過氧化物酶(MPO)活性,採用逆轉錄-聚閤酶鏈反應(RT-PCR)技術,對腸組織MIF的錶達水平進行半定量測定.結果 正常組DAI、MPO活性及MIFmRNA的錶達水平分彆為0.51±0.28、0.38±0.18、0.11±0.03;模型Ⅰ組5.04±0.73、1.68±0.45、0.65±0.04;模型Ⅱ組8.13±0.71、2.70±0.35、0.81±0.05.與正常組相比,模型組腸組織中MIFmR-NA的錶達顯著增彊(P<0.05),MPO活性及DAI也顯著增高(P<0.05),且在病情嚴重組增高尤為明顯.結論 大鼠結腸炎腸組織中MIF的錶達升高,併與疾病嚴重程度正相關,提示其可能與大鼠結腸炎的髮病有關.
목적 관찰거서세포이동억제인자(MIF)재삼초기분광산(TNBS)유발적대서결장염장조직중적표체급기여질병엄중정도적관계.방법 채용TNBS/을순용액관장제비대서결장염모형,24지동물분위정상조、경、중모형조공3조.매천관찰각조대서질병활동지수(DAI);생화법검측수과양화물매(MPO)활성,채용역전록-취합매련반응(RT-PCR)기술,대장조직MIF적표체수평진행반정량측정.결과 정상조DAI、MPO활성급MIFmRNA적표체수평분별위0.51±0.28、0.38±0.18、0.11±0.03;모형Ⅰ조5.04±0.73、1.68±0.45、0.65±0.04;모형Ⅱ조8.13±0.71、2.70±0.35、0.81±0.05.여정상조상비,모형조장조직중MIFmR-NA적표체현저증강(P<0.05),MPO활성급DAI야현저증고(P<0.05),차재병정엄중조증고우위명현.결론 대서결장염장조직중MIF적표체승고,병여질병엄중정도정상관,제시기가능여대서결장염적발병유관.
Objective To observe the expression of macrophage migration inhibitory factor (MIF) in colon tissues of rats with 2,4, 6-trinitrobenzene sulfonic acid (TNBS) induced colitis and the relationship between MIF and the severity of the colitis. Methods 24 Spra-gue-Dawley rats were randomly divided into three groups: normal group, model group Ⅰ and model group Ⅱ. Rat colitis model was induced by 2,4,6-trinitrobenzene sulfonic acid (TNBS), and disease activity index (DAI) was calculated every day. The myeloperoxidase (MPO) ac-tivity was detected by biochemistry, the expression of MIF in colon tissue was detected by semi-quantitative reverse transcriptional chain reac-tive (RT-PCR). Results In normal group, DAI, MPO activity and MIF mRNA level were 0.51±0. 28,0. 38±0. 18,0. 11±0. 03, while in model group Ⅰ were 5.04±0.73,1.68±0. 45,0. 65±0.04 and in model group Ⅱ were 8. 13±0.71,2. 70±0. 35,0. 81±0. 05. Com-pared with normal control, the expression of MIF mRNA significantly increased (P<0.05), MPO activity and DAI also significantly in-creased(P<0.05)in model group, especially in model group Ⅱ. Conclusion The expression of MIF increased in experimental colitis, and there was a positive relation between MIF expression and the severity of colitis, which suggested that MIF may be related to the pathogen-esis of TNBS-induced colitis.
