CAJ | 학술논문

应用免疫组织化学方法,检测Rap1GAP蛋白在甲状腺乳头状癌肿瘤组织及其癌旁组织中的表达情况,并运用甲基化特异性PCR方法进行Rap1GAP基因启动子区甲基化水平检测.结果显示,在69例检测样本中,有54例(78％)甲状腺乳头状癌患者的肿瘤组织Rap1GAP蛋白表达较癌旁组织下调；Rap1GAP蛋白的下调与患者的美国癌症联合委员会(AJCC)分期T值密切相关(P=0.043).甲基化分析发现,54例Rap1GAP表达下调的甲状腺乳头状癌中46例出现甲基化(66.7％),15例表达水平无明显变化的甲状腺乳头状癌中1例出现甲基化(6.67％),二者对比差异有统计学意义(P＜0.01)；同时69例癌旁正常组织未检测到甲基化.提示基因启动子区的甲基化改变可能是Rap1GAP蛋白表达下调的重要机制之一.
응용면역조직화학방법,검측Rap1GAP단백재갑상선유두상암종류조직급기암방조직중적표체정황,병운용갑기화특이성PCR방법진행Rap1GAP기인계동자구갑기화수평검측.결과현시,재69례검측양본중,유54례(78％)갑상선유두상암환자적종류조직Rap1GAP단백표체교암방조직하조；Rap1GAP단백적하조여환자적미국암증연합위원회(AJCC)분기T치밀절상관(P=0.043).갑기화분석발현,54례Rap1GAP표체하조적갑상선유두상암중46례출현갑기화(66.7％),15례표체수평무명현변화적갑상선유두상암중1례출현갑기화(6.67％),이자대비차이유통계학의의(P＜0.01)；동시69례암방정상조직미검측도갑기화.제시기인계동자구적갑기화개변가능시Rap1GAP단백표체하조적중요궤제지일.
The expression of Rap1 GAP protein was detected in 69 cases of papillary thyroid carcinoma and adjacent normal thyroid tissues with immunohistochemistry method.Methylation of Rapl GAP gene was analyzed by methylation-specific-PCR (MSP) in these tissues.The immunohistochemistry results indicated that the Rap1GAP protein was down-regulated in tumor tissues of 54 ( 78％ ) cases compared with normal thyroid tissues.Statistical analysis demonstrated that the decreased level of Rap1 GAP protein expression was significantly correlated with the tumor stage T according to American Joint Committee on Cancer ( AJCC,P =0.043 ).The MSP results demonstrated that 46 cases of Rap1GAP gene methylation were detected in 54 cases with down-regulated expression of Rap1GAP (66.7％),but only 1 case of methylation was found in 15 cases without obvious change of Rap1GAP expression (6.67％),showing significant difference ( P＜0.01 ).There was no methylation in normal thyroid tissues.These results suggest that raised methylation of promoter region may contribute to the low expression of Rap1GAP protein in papillary thyroid carcinoma.