CAJ | 학술논문

目的通过建立兔耳腹侧增生性瘢痕的模型,探讨6-O-羧甲基壳聚糖(carboxyl methyl chitosan,6-O-CM-CH)局部注射对瘢痕的作用.方法新西兰大白兔12只,在兔耳腹侧制备直径约1 cm、深达软骨膜的圆形瘢痕123个,随机将新西兰大白兔分为A、B、C3组,每组4只.分别于术后第30天、第40天瘢痕组织内注射药物,于术后第35天、第45天取瘢痕样本,分析成纤维细胞密度、羟脯氨酸含量和瘢痕增生指数.A组为治疗组,瘢痕内注射6-O-CM-CH(500μg/ml);B组为对照组1,注射曲安奈德(10 mg/ml);C组为对照组2,注射生理盐水.结果 A组与C组相比,瘢痕组织HE染色和胶原染色均可见胶原成纤维细胞密度降低(P＜0.05),羟脯氨酸含量降低(P＜0.05),瘢痕增生指数降低(P＜0.05),两组间差异有统计学意义;A组与B组相比较,成纤维细胞密度、羟脯氨酸含量、瘢痕增生指数差异均无统计学意义(P＞0.05).结论 6-O-CM-CH局部注射对兔耳增生性瘢痕的抑制作用与曲安奈德相近,局部注射能够抑制兔耳增生性瘢痕.
목적 통과건립토이복측증생성반흔적모형,탐토6-O-최갑기각취당(carboxyl methyl chitosan,6-O-CM-CH)국부주사대반흔적작용.방법 신서란대백토12지,재토이복측제비직경약1 cm、심체연골막적원형반흔123개,수궤장신서란대백토분위A、B、C3조,매조4지.분별우술후제30천、제40천반흔조직내주사약물,우술후제35천、제45천취반흔양본,분석성섬유세포밀도、간포안산함량화반흔증생지수.A조위치료조,반흔내주사6-O-CM-CH(500μg/ml);B조위대조조1,주사곡안내덕(10 mg/ml);C조위대조조2,주사생리염수.결과 A조여C조상비,반흔조직HE염색화효원염색균가견효원성섬유세포밀도강저(P＜0.05),간포안산함량강저(P＜0.05),반흔증생지수강저(P＜0.05),량조간차이유통계학의의;A조여B조상비교,성섬유세포밀도、간포안산함량、반흔증생지수차이균무통계학의의(P＞0.05).결론 6-O-CM-CH국부주사대토이증생성반흔적억제작용여곡안내덕상근,국부주사능구억제토이증생성반흔.
Objective To investigate the effect of carboxyl methyl chitosan on hypertrophic scars by establishing a hypertrophic scar model on the ventral side of rabbit ears.Methods Full-thick-ness excisional wounds,1 cm in diameter,were made in the ears of 12 adult New Zealand white rabbits,and 123 hypertrophic sears were made in all.Then the rabbits were divided into 3 groups:group A was an experimental group (carboxyl methyl chitosan,500μg/ml),group B was a control group 1 (triamcinolone),and group C was control group 2 (physiological saline).All the scars were injected with drugs on the 30th and 40th days after operation,and then the samples were collected on the 35th and 45th day and analyzed.Results Compared with group C,group A appeared to be flatter,softer,and lighter in color;the area density of fibroblast decreased using HE stain and masson stain (P<0.05),and hydroxyproline content and hypertrophic index were also lower than group C (P<0.05).There were no significant differences of those criteria between group A and group B (P>0.05).Conclusion Injection of carboxyl methyl chitosan into Iocal hypertrophic scars On rabbit ears has similar effects to triamcinolone,and both of them can prevent and cure hypertrophic scars in proliferative stage.