CAJ | 학술논문

目的：研究冠心病患者单核细胞凝血/纤溶活性改变与中医辨证分型的关系。方法： 80例冠心病患者分为血瘀证组30例，痰浊证组27例，气滞证组23例，另设健康对照组20例；采用发色底物法分别检测外周血单核细胞(PBMC)促凝活性(PCA)、组织型纤溶酶原激活物(t-PA)及其抑制物(PAI-1)活性。结果：冠心病各组与健康对照组比较，PBMC PCA、PAI-1表达均显著升高，而t-PA表达显著下降(P<0.01)；血瘀证与非血瘀证组(痰浊与气滞组)之间PBMC t-PA、PCA表达有显著差异(P<0.01)，而PAI-1表达无差异(P>0.05)；痰浊证组与气滞证组之间各项指标未见差异(P>0.05)。结论：冠心病患者单核细胞凝血/纤溶活性改变与中医辨证分型之间有一定的相关性，PBMC PCA增高和t-PA降低的程度对区别血瘀证和非血瘀证具有意义，血瘀证患者处于明显的血栓前状态。
목적： 연구관심병환자단핵세포응혈/섬용활성개변여중의변증분형적관계。방법： 80례관심병환자분위혈어증조30례，담탁증조27례，기체증조23례，령설건강대조조20례；채용발색저물법분별검측외주혈단핵세포(PBMC)촉응활성(PCA)、조직형섬용매원격활물(t-PA)급기억제물(PAI-1)활성。결과：관심병각조여건강대조조비교，PBMC PCA、PAI-1표체균현저승고，이t-PA표체현저하강(P<0.01)；혈어증여비혈어증조(담탁여기체조)지간PBMC t-PA、PCA표체유현저차이(P<0.01)，이PAI-1표체무차이(P>0.05)；담탁증조여기체증조지간각항지표미견차이(P>0.05)。결론：관심병환자단핵세포응혈/섬용활성개변여중의변증분형지간유일정적상관성，PBMC PCA증고화t-PA강저적정도대구별혈어증화비혈어증구유의의，혈어증환자처우명현적혈전전상태。
To study the relationship between changes of coagulant and fibrinolytic activity of human peripheral blood monocyte (PBMC) in coronary heart disease (CHD) patients and different syndrome-type of Traditional Chinese Medicine (TCM). Methods:80 patients with coronary heart disease were divided into three groups by syndrome differentiation. 30cases of blood stasis (BS), 27cases of phregm-damp(PD) and 23cases of Qi stagnation(QS). 20 healthy persons were enrolled as normal control group. Activity of tissue type plasminogen activator(t-PA) and its inhibitor (API-1) and procoagulant activity (PCA) of PBMC were measured by chromogenic substrate method respectively. Results: PAI-1 activity and PCA expression of PBMC in the CHD group were significantly higher while t-PA activity of PBMC was lower than those in the normal control group (P<0.01). PCA expression of PBMC in the BS group was significantly higher than that in PD and QS groups, PBMC t-PA expression in BS group was lower than that in PD and QS groups (P<0.01). No significant difference of PAI-1 activity of PBMC was found among BS、PD and QS groups (P>0.05). All param3eters were found no significant difference between PD and QS groups (P>0.05). Conclusions: There is a relationship between changes of coagulant and fibrinolytic activity of monocyte in coronary heart disease patients and different syndrome-type of TCM. The increasing level of PBMC PCA and reducing level of PBMC t-PA could be used as referential indices for differentiating BS and NBS(PD and QS). Patients of BS were in the notable prethrombotic state.