CAJ | 학술논문

使用离子交换和凝胶过滤色谱法从富硒螺旋藻中提纯含硒藻蓝蛋白.通过含硒藻蓝蛋白与藻蓝蛋白对4种不同的自由基清除能力的比较,即总抗氧化能力、清除DPPH自由基、过氧化物阴离子和引发红细胞溶血的过氧化氢自由基能力测定,从而对含硒藻蓝蛋白的抗氧化活性进行评价.结果显示,经提纯后的含硒藻蓝蛋白在某一特定剂量下与藻蓝蛋白在清除ABTS、DPPH、过氧化物阴离子和AAPH自由基能力相比,都表现出更好的抗氧化作用.评价提纯后的含硒藻蓝蛋白和藻蓝蛋白在抑制人体恶性黑素瘤细胞(A-375)的生长和增殖的作用.结果表明,提纯后的含硒藻蓝蛋白和藻蓝蛋白的IC50分别在44.5μM和65.9μM能显著地抑制A-375细胞的增殖.使用流体血细胞计数分析和末端脱氧核苷酸转移酶和脱氧尿三磷酸缺口末端标记对A-375细胞凋亡的诱发进行测定.
사용리자교환화응효과려색보법종부서라선조중제순함서조람단백.통과함서조람단백여조람단백대4충불동적자유기청제능력적비교,즉총항양화능력、청제DPPH자유기、과양화물음리자화인발홍세포용혈적과양화경자유기능력측정,종이대함서조람단백적항양화활성진행평개.결과현시,경제순후적함서조람단백재모일특정제량하여조람단백재청제ABTS、DPPH、과양화물음리자화AAPH자유기능력상비,도표현출경호적항양화작용.평개제순후적함서조람단백화조람단백재억제인체악성흑소류세포(A-375)적생장화증식적작용.결과표명,제순후적함서조람단백화조람단백적IC50분별재44.5μM화65.9μM능현저지억제A-375세포적증식.사용류체혈세포계수분석화말단탈양핵감산전이매화탈양뇨삼린산결구말단표기대A-375세포조망적유발진행측정.
Selenium-containing phycocyanin (Se-PC) was purified from Se-enriched Spirulina platensis using ion exchange and gel filtration chromatography. Antioxidant activity of Se-PC was investigated and compared with phycocyanin (PC) by using four different free radical scavenging assays, namely, trolox equivalent antioxidant capacity (TEAC) assay, DPPH free radical scavenging assay, superoxide anion scavenging assay and assay for erythrocyte hemolysis mediated by peroxyl free radicals (AAPH assay) . The results indicated that the purified Se-PC exhibited better antioxidant activities than PC by scavenging the ABTS, DPPH, superoxide anion and AAPH free radicals in a dose-dependent manner. The anticancer activities of purified Se-PC and PC were tested on growth and multiplication of human malignant melanoma cell line (A-375) . The results indicate that Se-PC and PC significantly inhibit the proliferation of A-375 cells with IC50 values at 44.5 μ M and 65.9 μ M respectively. The induction of apoptosis in A-375 cells was evaluated by using flow cytometric analysis and the terminal deoxynucleotidyltransferase - mediated dUTP nick end labeling assay.