CAJ | 학술논문

为加强对密云水库水体状况的全面了解,对2006年11月到2007年11月不同季节水体中的微生物进行了研究.用流式细胞仪技术和LB固体培养基培养测定了水体中的细菌数目;利用PCR-DGGE技术对水体细菌的多样性和相似性进行分析.结果表明,细菌数目随季节变化有所不同,但利用流式细胞仪测定的每毫升水体细菌的数目基本上均为104数量级,而培养得到的细菌菌落数为102～103 CFU/ml,基本符合国家饮用水源的卫生标准.细菌的多样性指数也表现出随季节变化的规律,冬季(2006-11和2007-11)Shannon-Weaver多样性指数较低,为3.0左右;春季(2007-04)开始增大,至夏季(2007-07)时可高达3.3068;秋季(2007-09)又减小到3.1246.另外,密云水库细菌的优势类群有β-Proteobacteria、Actinobacteria、Cytophaga/Flexibacter/Bacteroides (CFB) 类群和Cyanobacteria. 这些资料有望为以后进一步从微生物角度评价水质提供参考.
위가강대밀운수고수체상황적전면료해,대2006년11월도2007년11월불동계절수체중적미생물진행료연구.용류식세포의기술화LB고체배양기배양측정료수체중적세균수목;이용PCR-DGGE기술대수체세균적다양성화상사성진행분석.결과표명,세균수목수계절변화유소불동,단이용류식세포의측정적매호승수체세균적수목기본상균위104수량급,이배양득도적세균균락수위102～103 CFU/ml,기본부합국가음용수원적위생표준.세균적다양성지수야표현출수계절변화적규률,동계(2006-11화2007-11)Shannon-Weaver다양성지수교저,위3.0좌우;춘계(2007-04)개시증대,지하계(2007-07)시가고체3.3068;추계(2007-09)우감소도3.1246.령외,밀운수고세균적우세류군유β-Proteobacteria、Actinobacteria、Cytophaga/Flexibacter/Bacteroides (CFB) 류군화Cyanobacteria. 저사자료유망위이후진일보종미생물각도평개수질제공삼고.
In this study, we evaluated the bacterial populations in Miyun Reservoir in different seasons from Nov. 2006 to Nov. 2007 to determine the overall water quality of the reservoir. The bacterial counts in water samples collected from the reservoir were determined by flow cytometry and by culture in LB medium. In addition, the bacterial diversity and dominant groups were also evaluated by PCR-DGGE. The results revealed that the bacterial counts were different according to the season, but that the average counts, which were determined to be 104 per ml by flow cytometry and 102-103 CFU per ml by culture in LB medium, were below the national standards for resource water. In addition, determination of the bacterial Shannon-Weaver diversity index revealed that the diversity also varied by season. Specifically, diversity was very low in winter (approximately 3.0 in November 2006 and 2007), but increased to as high as 3.3068 in the spring and summer (April and July, 2007), after which it began to decrease, with a value of 3.1246 being observed in autumn (September 2007). Furthermore, the dominant groups of bacteria observed in Miyun Reservoir included β-Proteobacteria, Actinobacteria, Cytophaga/Flexibacter/Bacteroides (CFB) phylum and Cyanobacteria. Taken together, the results of this study will help to understand future changes in the water quality of Miyun Reservior.