CAJ | 학술논문

从处理硫酸盐废水厌氧折流板反应器(Anaerobic baffle reactor,ABR)的污泥中分离到1株硫酸盐还原菌,对该菌株进行了形态、生理生化特性方面的研究,并对165 rDNA序列进行了分析.该菌株为杆状或弧状,大小为(0.5～0.7)μm×(1.4～1.9)μm,革兰染色阴性,芽胞染色阴性,能运动,具有硫酸盐还原功能.菌株最适生长pH为7.0～8.0,喜中性偏碱环境;初始[SO_4~(2-)]为2 000 mg/L,OD_(600nm)值为1.206,SO_4~(2-)去除率达到71%:该菌株能分别利用乳酸、丙酮酸、丁酸、乙酸、乙醇、甲醇、葡萄糖作为电子供体.进行硫酸盐异化还原,乳酸最有利于该菌SO_4~(2-)的去除,SO_4~(2-)去除率为91.4%,其次为丙酮酸,达到51.2%.基于16S rDNA序列同源性构建了系统发育树,结果表明此株菌是属于脱硫弧菌属(Desulfovibrio)的硫酸盐还原菌,与Desulfovibrio具有96.0%的序列相似性.
종처리류산염폐수염양절류판반응기(Anaerobic baffle reactor,ABR)적오니중분리도1주류산염환원균,대해균주진행료형태、생리생화특성방면적연구,병대165 rDNA서렬진행료분석.해균주위간상혹호상,대소위(0.5～0.7)μm×(1.4～1.9)μm,혁란염색음성,아포염색음성,능운동,구유류산염환원공능.균주최괄생장pH위7.0～8.0,희중성편감배경;초시[SO_4~(2-)]위2 000 mg/L,OD_(600nm)치위1.206,SO_4~(2-)거제솔체도71%:해균주능분별이용유산、병동산、정산、을산、을순、갑순、포도당작위전자공체.진행류산염이화환원,유산최유리우해균SO_4~(2-)적거제,SO_4~(2-)거제솔위91.4%,기차위병동산,체도51.2%.기우16S rDNA서렬동원성구건료계통발육수,결과표명차주균시속우탈류호균속(Desulfovibrio)적류산염환원균,여Desulfovibrio구유96.0%적서렬상사성.
A strain of sulfate-reducing bacterium was isolated from the sewage of an anaerobic baffle reactor (ABR). The morphological and biochemical characteristics as well as 16S rDNA sequence of the strain was studied. The cells are vibrioid-shaped or rod shape with the size of (0.5 ～ 0.7) μm × (1.4 ～ 1.9) μm. The strain is gram negative, motile and doesn' t form spore. The best pH value for growth is 7.0 -8.0. When the initial [SO_4~(2-)]is 2 000 mg/L, the highest OD_(600nm) is 1. 206 and the SO_4~(2-) removal efficiency is up to 71%. The strain can use lactic acid, pyruvatic acid, butyric acid, acetic acid, ethanol, methanol and glucose as electron donor respectively. Lactic acid is the most suitable electron donor to reduce SO_4~(2-) , the SO_4~(2-) removal efficiency is up to 91.4% when lactic acid is the sole car-bon source. The results of 16S rRNA sequence and phylogenetic analysis show that the strain is the closest relative to Desulfovibrio sp. With 96.0% sequence similarity.