CAJ | 학술논문

目的:研究补气温阳、活血化瘀中药复方(组成:人参、黄芪、车前子、苏木、川芎、丹参、制附子、枳壳、桂枝)对大鼠充血性心力衰竭的影响机制.方法:以大鼠腹腔注射盐酸阿霉素(ADR)制造充血性心衰动物模型,分为对照组、ADR心力衰竭模型组、心宝治疗组、活血温阳益气复方治疗组,并分别以生理盐水、心宝药液、活血温阳益气复方药液灌胃干预治疗,使用放免法、半定量逆转录-聚合酶链反应法(RT-PCR)及流式细胞技术测定心衰大鼠治疗前后血浆心钠素(ANP)、肾髓质精氨酸血管加压素依赖性水通道蛋白-2(AQP2)及热休克蛋白70(HSP70)的表达水平差异.结果:活血温阳益气复方可显著提高心衰大鼠的心输出量、左室收缩内压,降低左心室舒张末压,改善左室内压最大变化速率,从而有效地改善了心衰大鼠的血流动力学;同时,该方能够显著降低心衰大鼠血浆ANP的水平和纠正心衰大鼠肾髓质AQP2蛋白的异常表达,并可提高白细胞中HSP70的转录和翻译水平.结论:该活血温阳益气复方能有效减轻大鼠充血性心力衰竭的程度,其机制可能与其减少血浆ANP水平、矫正肾脏AQP2异常表达和增强 HSP70表达有关.
목적:연구보기온양、활혈화어중약복방(조성:인삼、황기、차전자、소목、천궁、단삼、제부자、지각、계지)대대서충혈성심력쇠갈적영향궤제.방법:이대서복강주사염산아매소(ADR)제조충혈성심쇠동물모형,분위대조조、ADR심력쇠갈모형조、심보치료조、활혈온양익기복방치료조,병분별이생리염수、심보약액、활혈온양익기복방약액관위간예치료,사용방면법、반정량역전록-취합매련반응법(RT-PCR)급류식세포기술측정심쇠대서치료전후혈장심납소(ANP)、신수질정안산혈관가압소의뢰성수통도단백-2(AQP2)급열휴극단백70(HSP70)적표체수평차이.결과:활혈온양익기복방가현저제고심쇠대서적심수출량、좌실수축내압,강저좌심실서장말압,개선좌실내압최대변화속솔,종이유효지개선료심쇠대서적혈류동역학;동시,해방능구현저강저심쇠대서혈장ANP적수평화규정심쇠대서신수질AQP2단백적이상표체,병가제고백세포중HSP70적전록화번역수평.결론:해활혈온양익기복방능유효감경대서충혈성심력쇠갈적정도,기궤제가능여기감소혈장ANP수평、교정신장AQP2이상표체화증강 HSP70표체유관.
AIM: To study the effects of Huoxue Huayu and Yiqi Wenyang compound prescription (composition; ginseng, radix astragali, plantaginis semen, lignum sappan, chuanxiong, salvia miltiorrhiza, radix aconiti lateralis preparata, bitter orange, cassia twig) of TCM on the congestive heart failure in rats. METHODS:The rats were given doxorubicin hydrochloride by intraperitoneal injection to establish the model of congestive heart failure and were randomly divided into control group, congestive heart failure model group, Xinbao treatment group ( XB treatment) and Huoxue Huayu and Yiqi Wenyang compound prescription treatment group (QXYTM treatment). Normal saline, liquid medicine Xinbao and liquid medicine Huoxue Huayu and Yiqi Wenyang compound prescription were administered respectively to four groups by gavage. Radioimmunoassay, semi - quantitative RT - PCR and flow cytometry methods were used to determine the expressions of plasma atrial natriuretic peptide ( ANP) , renal medulla aquaporin - 2 ( AQP2 ) and heat shock protein70 (HSP70) in congestive heart failure rats before and after treatment. RESULTS: In congestive heart failure rats treated with Huoxue Huayu and Yiqi Wenyang compound prescription, the cardiac output and left ventricle systolic pressure increased significantly, the left ventricular end diastolic pressure decreased, and the maximum ascending and declining rate of left ventricular pressure ameliorated, thus the hemodynamics was effectively improved. Meanwhile, the level of plasma cardionatrin decreased, the abnormal expression of renal medulla AQP2 was markedly restored, and the transcription and expression of HSP70 gene were also increased. CONCLUSION: Huoxue Huayu and Yiqi Wenyang compound prescription promotes the recovery of the pathogenetic conditions of congestive heart failure in rare. The mechanism may be in association with a decrease in plasma ANP level, correction of renal medulla AQP2 abnormal expression and enhancement of HSP70 expression.