上海交通大学学报（医学版）
上海交通大學學報（醫學版）
상해교통대학학보（의학판）
JOURNAL OF SHANGHAI JIAOTONG UNIVERSITY(MEDICAL SCIENCE)
2010年
2期
158-161
,共4页
拓扑替康%顺铂%泰素%HeLa细胞%放射增敏
拓撲替康%順鉑%泰素%HeLa細胞%放射增敏
탁복체강%순박%태소%HeLa세포%방사증민
Topotecan%Cisplatin%Taxol%HeLa cell line%radiosensitization
目的 研究拓扑替康(TPT)对宫颈癌HeLa细胞的杀伤作用和放射增敏作用,并与顺铂(DDP)和泰素(TAX)进行对比分析.方法 采用MTT法检测TPT、DDP和TAX对宫颈癌HeLa细胞增殖能力的影响,细胞克隆形成法研究三种化疗药物的放射增敏作用,并应用单击多靶模型计算放射增敏比.结果 TPT、DDP和TAX作用HeLa细胞24、48和72 h半数抑制浓度分别为8.0、2.6和0.8 μg/mL,2.4、0.7和0.1μg/mL,0.3、0.1和0.0μg/mL.三种化疗药物的放射增敏组肿瘤细胞凋亡率均明显高于单放射组和单独化疗组(P<0.05);放射增敏实验中,TPT作用24 h和48 h后放射增敏比分别为1.167和1.344,DDP为1.314和1.538,TAX为1.076和1.316.结论 TPT、DDP和TAX对宫颈癌HeLa细胞具有明显抑制作用,且呈时间和浓度依赖性.三种化疗药物具有放射增敏作用,DDP的放射增敏作用最强,TAX最弱.
目的 研究拓撲替康(TPT)對宮頸癌HeLa細胞的殺傷作用和放射增敏作用,併與順鉑(DDP)和泰素(TAX)進行對比分析.方法 採用MTT法檢測TPT、DDP和TAX對宮頸癌HeLa細胞增殖能力的影響,細胞剋隆形成法研究三種化療藥物的放射增敏作用,併應用單擊多靶模型計算放射增敏比.結果 TPT、DDP和TAX作用HeLa細胞24、48和72 h半數抑製濃度分彆為8.0、2.6和0.8 μg/mL,2.4、0.7和0.1μg/mL,0.3、0.1和0.0μg/mL.三種化療藥物的放射增敏組腫瘤細胞凋亡率均明顯高于單放射組和單獨化療組(P<0.05);放射增敏實驗中,TPT作用24 h和48 h後放射增敏比分彆為1.167和1.344,DDP為1.314和1.538,TAX為1.076和1.316.結論 TPT、DDP和TAX對宮頸癌HeLa細胞具有明顯抑製作用,且呈時間和濃度依賴性.三種化療藥物具有放射增敏作用,DDP的放射增敏作用最彊,TAX最弱.
목적 연구탁복체강(TPT)대궁경암HeLa세포적살상작용화방사증민작용,병여순박(DDP)화태소(TAX)진행대비분석.방법 채용MTT법검측TPT、DDP화TAX대궁경암HeLa세포증식능력적영향,세포극륭형성법연구삼충화료약물적방사증민작용,병응용단격다파모형계산방사증민비.결과 TPT、DDP화TAX작용HeLa세포24、48화72 h반수억제농도분별위8.0、2.6화0.8 μg/mL,2.4、0.7화0.1μg/mL,0.3、0.1화0.0μg/mL.삼충화료약물적방사증민조종류세포조망솔균명현고우단방사조화단독화료조(P<0.05);방사증민실험중,TPT작용24 h화48 h후방사증민비분별위1.167화1.344,DDP위1.314화1.538,TAX위1.076화1.316.결론 TPT、DDP화TAX대궁경암HeLa세포구유명현억제작용,차정시간화농도의뢰성.삼충화료약물구유방사증민작용,DDP적방사증민작용최강,TAX최약.
Objective To investigate the inhibition and radiosensitization effects of Topotecan(TPT)on cervical cancer cell line HeLa,and make comparison with Cisplatin(DDP)and Taxol(TAX).Methods The effects of TPT,DDP and TAX on proliferation of cervical cancer cell line HeLa were evaluated by MTT assay.The radiosensitization effects of TPT,DDP and TAX on HeLa cells were detected by clone formation assay,and the sensitization enhancement ratios of TPT,DDP and TAX were calculated.Results The IC_(50) of TPT at different reaction time(24 h,48 h and 72 h)were 8.0μg/mL,2.6 μg/mL and 0.8 μg/mL,respectively,those of DDP were 2.4 μg/mL,0.7 μg/mL and 0.1μg/mL,respectively,and those of TAX were 0.3 μg/mL,0.1μg/mL and 0.0 μg/mL, respectively.The apoptosis rates of tumor cells treated by radiosensitization were significantly higher than those of tumor cells treated by single radiotherapy or single chemotherapy (P<0.05).The sensitization enhancement ratios of TPT at 24 h and 48 h were 1.167 and 1.344,respectively,those of DDP were 1.314 and 1.538,respectively,and those of TAX were 1.076 and 1.316,respectively.Conclusio TPT,DDP and TAX have significant inhibition effects on cervical cancer cell line HeLa, which are time-dependant and dose-dependant. Besides,TPT,DDP and TAX have radiosensitizing effects, with DDP being the strongest and TAX being the weakest.
