CAJ | 학술논문

本文根据截叶苜蓿(Medicago trucatula)几丁质酶ClassⅢ基因(GenBank:AY294484.1)的全长序列设计引物,通过RT-PCR的方法得到紫花苜蓿(Medicago sative)几丁质酶ClassⅢ的核酸序列,命名为MsChiⅢ,在NCBI中的登录号为FJ872918.利用生物信息学软件分析MsChiⅢ,预测氨基酸序列的等电点、二级结构和三级结构,并构建系统发育树.结果显示该核酸序列全长为953 bp,包括完整的开放阅读框,编码301个氨基酸,等电点为8.212.该序列所编码的蛋白属于几丁质酶18家族的内切酶,分布于细胞间隙,并含有几丁质酶18家族的特征序列--LGDVDFDIE,并预测MschiⅢ可能是一种具有几丁质酶和溶菌酶的双功能酶.
본문근거절협목숙(Medicago trucatula)궤정질매ClassⅢ기인(GenBank:AY294484.1)적전장서렬설계인물,통과RT-PCR적방법득도자화목숙(Medicago sative)궤정질매ClassⅢ적핵산서렬,명명위MsChiⅢ,재NCBI중적등록호위FJ872918.이용생물신식학연건분석MsChiⅢ,예측안기산서렬적등전점、이급결구화삼급결구,병구건계통발육수.결과현시해핵산서렬전장위953 bp,포괄완정적개방열독광,편마301개안기산,등전점위8.212.해서렬소편마적단백속우궤정질매18가족적내절매,분포우세포간극,병함유궤정질매18가족적특정서렬--LGDVDFDIE,병예측MschiⅢ가능시일충구유궤정질매화용균매적쌍공능매.
The eDNA of chitinase Class Ⅲ gene (GenBank accession:AY294484.1) from Medicago sative was cloned by RT-PCR,with primers designed according to the sequence of chitinase Class Ⅲ of Medicago trucatula,It was named as MsChi Ⅲ,and its GenBank accession number is FJ872918.The isoelectric point of amino acid sequence,secondary and three-dimersional structures of MsChi Ⅲ were analysed by bioinformatics software,and the homologue tree of chitinase Ⅲ was established.The result showed that the sequence consisted of 953 bp and had an full open reading frame,which encoded a polypeptide of 301 amino acid residues.The estimated PI of Class Ⅲ chitinase of Medicago satire was 8.212.The protein coded by this sequence as incision enzyme belong to chitinase family 18,dispersed in intercellular space,and the amino acid sequence included special sequence of chitinase family 18,with an amino acid sequence of LGDVDFDIE.The effects of chitinase Class Ⅲ would include chitinase and lysozyme.