CAJ | 학술논문

采用活性跟踪的方法分离纯化野生红豆越橘提取物，并将有效组分进行抗癌细胞增殖活性测定。首先采用大孔吸附树脂X-5富集分离红豆越橘丙酮提取物，多酚含量和ABTS抗氧化做指标确定乙醇梯度洗脱有效组分，并命名为LB-1、LB-2、LB-3、LB-4，其总洗脱物命名为LB-MIX。用MTT法检测以上5个组分抗结肠癌HT29、卵巢癌Hela、肺癌A549和肝癌HepG-2细胞增殖活性，并选用环磷酰胺做阳性对照。结果显示，LB-MIX具有较好的抑制几株癌细胞的增殖活性。
채용활성근종적방법분리순화야생홍두월귤제취물，병장유효조분진행항암세포증식활성측정。수선채용대공흡부수지X-5부집분리홍두월귤병동제취물，다분함량화ABTS항양화주지표학정을순제도세탈유효조분，병명명위LB-1、LB-2、LB-3、LB-4，기총세탈물명명위LB-MIX。용MTT법검측이상5개조분항결장암HT29、란소암Hela、폐암A549화간암HepG-2세포증식활성，병선용배린선알주양성대조。결과현시，LB-MIX구유교호적억제궤주암세포적증식활성。
In this experiment, purified extracts of Vaccinium vitis-idaea with the method of tracking activity, and determine the main anthocyanin composition by liquid chromatography-mass spectrometry techniques. First, enrichment and separation of acetone extracts of Vaccinium vitis-idaea by macroporous resin X-5, and determine the effective components of alcohol gradient by the phenolic contentation and scavenging activity of ABTS radical, and named LB-1, LB-2, LB-3, LB-4, the total eluate named LBMIX. The inhibition of HT29 colon cancer, Hela cervical cancer, A549 lung cancer, and HepG-2 liver cancer cell proliferation of five fractions were employed by MTT assay. The results showed that LB-MIX has a better inhibition of the four cancer cells proliferation activity.