现代农业科技
現代農業科技
현대농업과기
XIANDAIHUA NONGYE
2012年
3期
35-36,39
,共3页
周英%高紫兰%袁凌南%周家春
週英%高紫蘭%袁凌南%週傢春
주영%고자란%원릉남%주가춘
粪肠球菌%F71%增殖%培养基%优化%活菌数
糞腸毬菌%F71%增殖%培養基%優化%活菌數
분장구균%F71%증식%배양기%우화%활균수
Enterococcus faecalis%F17%multiplication%medium%optimazation%viable count
为达到增殖菌体的目的，对粪肠球菌F71进行了培养基优化正交试验。结果表明，最佳培养基的组成为：胰蛋白胨10 g/L，酵母粉10 g/L，葡萄糖 10 g/L，无水氯化钙0.04 g/L，七水合硫酸镁0.019 2 g/L，磷酸氢二钾0.04 g/L，磷酸二氢钾0.04 g/L，磷酸氢钠0.4 g/L，氯化钠0.08 g/L，土豆汁50 g/L，碳酸钙1 g/L，乙酸钠10 g/L，碳酸铵2 g/L，乙酸铵1 g/L，半胱氨酸盐酸盐0.5 g/L，pH值为9，最适培养温度为35 ℃。用此培养基进行增殖，F71的最高活菌数达到2.78×108 cfu/mL，比基础培养基提高了54.6%。
為達到增殖菌體的目的，對糞腸毬菌F71進行瞭培養基優化正交試驗。結果錶明，最佳培養基的組成為：胰蛋白胨10 g/L，酵母粉10 g/L，葡萄糖 10 g/L，無水氯化鈣0.04 g/L，七水閤硫痠鎂0.019 2 g/L，燐痠氫二鉀0.04 g/L，燐痠二氫鉀0.04 g/L，燐痠氫鈉0.4 g/L，氯化鈉0.08 g/L，土豆汁50 g/L，碳痠鈣1 g/L，乙痠鈉10 g/L，碳痠銨2 g/L，乙痠銨1 g/L，半胱氨痠鹽痠鹽0.5 g/L，pH值為9，最適培養溫度為35 ℃。用此培養基進行增殖，F71的最高活菌數達到2.78×108 cfu/mL，比基礎培養基提高瞭54.6%。
위체도증식균체적목적，대분장구균F71진행료배양기우화정교시험。결과표명，최가배양기적조성위：이단백동10 g/L，효모분10 g/L，포도당 10 g/L，무수록화개0.04 g/L，칠수합류산미0.019 2 g/L，린산경이갑0.04 g/L，린산이경갑0.04 g/L，린산경납0.4 g/L，록화납0.08 g/L，토두즙50 g/L，탄산개1 g/L，을산납10 g/L，탄산안2 g/L，을산안1 g/L，반광안산염산염0.5 g/L，pH치위9，최괄배양온도위35 ℃。용차배양기진행증식，F71적최고활균수체도2.78×108 cfu/mL，비기출배양기제고료54.6%。
In order to realize the multiplication of isolates,the optimization experiment of medium for Enterococcusfaecalis F71 was carried out by orthogonal design. The optimization of cultured medium of F71 was as follows：peptone 10 g/L,yeast extract l0 g/L,glucose 10 g/L, anhydrous calcium chloride 0.04 g/L,bitter salt 0.019 2 g/L,dipotassium hydrogen phosphate 0.04 g/L ,monopotassium phosphate 0.04 g/L,monosodium phosphate 0.4 g/L,sodium chloride 0.08 g/L,juice of potato 50 g/L,calcium carbonate 1 g/L,sodium acetate 10 g/L,ammonium carbonate 2 g/L,ammonium acetate 1 g/L, cysteine hydrochloride 0.5 g/L. The optimal initial pH value of the medium was 9, F71 got to 2.78× 10^8 cfu/mL in this culture for 16 hours at 35 ℃ ,and increased by 54.6% compared to the basic culture medium.
