CAJ | 학술논문

目的探讨外用他克莫司对寻常型银屑病患者外周血CD4+CD25+调节性T细胞(CD4+CD25+Treg)的影响.方法给予48例寻常型银屑病患者他克莫司软膏,每日2次外用,用药8周.治疗前和治疗后,用流式细胞仪检测患者外周血CD4+CD25+调节性T细胞的表达水平,并对患者进行PASI评分.结果治疗8周后,总有效率为91.5%.治疗前,银屑病进行期、静止期患者及正常人外周血CD4+CD25+Treg与CD4+淋巴细胞的百分比为(2.64±0.86)%、(3.98±0.96)%、(8.46±1.54)%,三者比较,差异均有极显著性(P＜0.01);治疗后,进行期和静止期患者外周CD4+CD25+Treg与CD4+淋巴细胞的百分比较治疗前均有明显升高(P＜0.01),但仍低于正常人,三者比较,差异均有极显著性(P＜0.01).结论寻常型银屑病存在CD4+CD25+调节性T细胞异常,他克莫司可通过上调CD4+CD25+调节性T细胞来治疗寻常型银屑病.
목적 탐토외용타극막사대심상형은설병환자외주혈CD4+CD25+조절성T세포(CD4+CD25+Treg)적영향.방법 급여48례심상형은설병환자타극막사연고,매일2차외용,용약8주.치료전화치료후,용류식세포의검측환자외주혈CD4+CD25+조절성T세포적표체수평,병대환자진행PASI평분.결과 치료8주후,총유효솔위91.5%.치료전,은설병진행기、정지기환자급정상인외주혈CD4+CD25+Treg여CD4+림파세포적백분비위(2.64±0.86)%、(3.98±0.96)%、(8.46±1.54)%,삼자비교,차이균유겁현저성(P＜0.01);치료후,진행기화정지기환자외주CD4+CD25+Treg여CD4+림파세포적백분비교치료전균유명현승고(P＜0.01),단잉저우정상인,삼자비교,차이균유겁현저성(P＜0.01).결론 심상형은설병존재CD4+CD25+조절성T세포이상,타극막사가통과상조CD4+CD25+조절성T세포래치료심상형은설병.
Objective To explore the effect of tacrolimus onitment on the changes of CD4+ and CD25+ Treg cells in peripheral blood from patients with psoriasis vulgaris. Methods 48 patients with psoria-sis vulgaris were treated with a topical immunomodulator, tacrolimus ointment 0.1%, twice daily for 8 weeks.The proportion of CD4+ and CD25+ Treg cells population in total T cells were evaluated by flowing cytometry before and after treatment. PASI was measured in all the patients. Results After 8-week treatment, the response rates was 91.5%. Before treatment, in the active stage and resting stage of psoriasis vulgaris and healthy controls, the proportion of CD4+ and CD25+ Treg cells population in total T cells was (2.64 ± 0.86) %,(3.98 ± 0.96) %, and (8.46 ± 1.54) %, with a significant difference among the three groups (P<0.01). After treatment, the proportion of CD4+ and CD25+ Treg cells population was obviously increased, but still lower than that in the healthycontrols (P<0.O1). Conclusions CD4+ and CD25+ Treg cells are abnormal in patients with psoriasis vulgaris. Tacrolimus can treat psoriasis vulgaris by upregulating CD4+ and CD25+ Treg cells.