中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2012年
3期
284-287
,共4页
林群%雷立华%曾邦雄%林献忠%林财珠%郑辉哲%杨庆%蔡宏达%高友光%林健清
林群%雷立華%曾邦雄%林獻忠%林財珠%鄭輝哲%楊慶%蔡宏達%高友光%林健清
림군%뢰립화%증방웅%림헌충%림재주%정휘철%양경%채굉체%고우광%림건청
肝细胞生长因子%原癌基因蛋白质c-met%高血压,肺性
肝細胞生長因子%原癌基因蛋白質c-met%高血壓,肺性
간세포생장인자%원암기인단백질c-met%고혈압,폐성
Hepatocyte growth factor%Proto-oncogene proteins c-met%Hypertension,pulmonary
目的 观察肺动脉高压大鼠肺内肝细胞生长因子(HGF)及其受体(c-met)表达的变化.方法 雄性SD大鼠80只,7周龄,体重180～250 g,采用随机数字表法,将其随机分为2组(n=40):对照组(C组)和肺动脉高压组(PH组).PH组经左侧开胸行左肺切除术,手术结束后关胸,待自主呼吸恢复后拔除气管导管,2周后颈部皮下注射野百合碱60 mg/kg,制备肺动脉高压模型；C组仅开胸,2周后颈部皮下注射等容量生理盐水.分别于注射野百合碱前1 d(基础值)、注射野百合碱后7、14、21和28 d时,各取8只大鼠,监测肺动脉压(mPAP),计算右心室与左心室+室间隔质量比[RV/( LV+S)],计算肌型肺小动脉血管中膜相对厚度(RWT1和RWT分别表示直径为50～100 μm和100-150μm的血管中膜相对厚度).采用RT-PCR法检测肺组织HGF mRNA和c-met mRNA的表达,采用Western blot法检测肺组织HGF蛋白和c-met蛋白的表达,采用ELISA法检测肺组织转化生长因子-β(TGF-β)含量.结果 与C组比较,PH组注射野百合碱后14、21和28 d时mPAP、RV/ (LV+S)和RWT2升高,HGF蛋白表达下调,注射野百合碱后7、14、21和28 d时RWT1升高,肺组织HGF mRNA表达下调,TGF-β含量升高(P＜0.01),c-met mRNA及其蛋白表达差异无统计学意义(P＞0.05).结论 肺组织HGF合成不足可能参与了肺动脉高压的形成,其机制与肺组织TGF-β含量升高有关.
目的 觀察肺動脈高壓大鼠肺內肝細胞生長因子(HGF)及其受體(c-met)錶達的變化.方法 雄性SD大鼠80隻,7週齡,體重180～250 g,採用隨機數字錶法,將其隨機分為2組(n=40):對照組(C組)和肺動脈高壓組(PH組).PH組經左側開胸行左肺切除術,手術結束後關胸,待自主呼吸恢複後拔除氣管導管,2週後頸部皮下註射野百閤堿60 mg/kg,製備肺動脈高壓模型；C組僅開胸,2週後頸部皮下註射等容量生理鹽水.分彆于註射野百閤堿前1 d(基礎值)、註射野百閤堿後7、14、21和28 d時,各取8隻大鼠,鑑測肺動脈壓(mPAP),計算右心室與左心室+室間隔質量比[RV/( LV+S)],計算肌型肺小動脈血管中膜相對厚度(RWT1和RWT分彆錶示直徑為50～100 μm和100-150μm的血管中膜相對厚度).採用RT-PCR法檢測肺組織HGF mRNA和c-met mRNA的錶達,採用Western blot法檢測肺組織HGF蛋白和c-met蛋白的錶達,採用ELISA法檢測肺組織轉化生長因子-β(TGF-β)含量.結果 與C組比較,PH組註射野百閤堿後14、21和28 d時mPAP、RV/ (LV+S)和RWT2升高,HGF蛋白錶達下調,註射野百閤堿後7、14、21和28 d時RWT1升高,肺組織HGF mRNA錶達下調,TGF-β含量升高(P＜0.01),c-met mRNA及其蛋白錶達差異無統計學意義(P＞0.05).結論 肺組織HGF閤成不足可能參與瞭肺動脈高壓的形成,其機製與肺組織TGF-β含量升高有關.
목적 관찰폐동맥고압대서폐내간세포생장인자(HGF)급기수체(c-met)표체적변화.방법 웅성SD대서80지,7주령,체중180～250 g,채용수궤수자표법,장기수궤분위2조(n=40):대조조(C조)화폐동맥고압조(PH조).PH조경좌측개흉행좌폐절제술,수술결속후관흉,대자주호흡회복후발제기관도관,2주후경부피하주사야백합감60 mg/kg,제비폐동맥고압모형；C조부개흉,2주후경부피하주사등용량생리염수.분별우주사야백합감전1 d(기출치)、주사야백합감후7、14、21화28 d시,각취8지대서,감측폐동맥압(mPAP),계산우심실여좌심실+실간격질량비[RV/( LV+S)],계산기형폐소동맥혈관중막상대후도(RWT1화RWT분별표시직경위50～100 μm화100-150μm적혈관중막상대후도).채용RT-PCR법검측폐조직HGF mRNA화c-met mRNA적표체,채용Western blot법검측폐조직HGF단백화c-met단백적표체,채용ELISA법검측폐조직전화생장인자-β(TGF-β)함량.결과 여C조비교,PH조주사야백합감후14、21화28 d시mPAP、RV/ (LV+S)화RWT2승고,HGF단백표체하조,주사야백합감후7、14、21화28 d시RWT1승고,폐조직HGF mRNA표체하조,TGF-β함량승고(P＜0.01),c-met mRNA급기단백표체차이무통계학의의(P＞0.05).결론 폐조직HGF합성불족가능삼여료폐동맥고압적형성,기궤제여폐조직TGF-β함량승고유관.
Objective To investgate the changes in the expression of hepatocyte growth factor (HGF)and c-met in the lungs in a rat model of pulmonary hypertension.Methods Eighty 7 week old male SD rats weighing 180-250 g were randomly divided into 2 groups ( n =40 each ):control group (group C) and pulmonary hypertension group (group PH).Pulmonary hypertension was induced by left pneumonectomy and subcutaneous monocrotaline (MCT) 60 mg/kg 2 weeks later.Pulmonary artery pressure and the ratio between the weight of right ventricle and left ventricle + interventricular septum ( RV/LV + S) were measured at 7,14,21 and 28 d after MCT administration.HGF and c-met protein and mRNA expression and TGF-β content in the lung tissue were determined.Results Pulmonary hypertension and right ventricular hypertrophy associated with hypertrophy of pulmonary artery tunica media and muscularization of small pulmonary arteries developed after MCT administration in PH group.In PH group HGF protein and mRNA expression in the lungs was significantly down-regulated as compared with group C.There were no significant differences in c-met protein and mRNA expression in the lungs between the 2 groups.The TGF-β content in the lungs was significantly increased in group PH as compared with group C.Conclusion Decrease in HGF production in the lungs plays an important role in the pulmonary hypertension.Increasing of pulmonary TGF-β may play an important role in the down-regulation of pulmonary HGF expression during pulmonary hypertension.
