中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2010年
5期
468-470
,共3页
吴波%李薇%喻良文%付玉梅
吳波%李薇%喻良文%付玉梅
오파%리미%유량문%부옥매
参环毛蚓%镉%溶酶体%土壤污染物%中性红保留时间
參環毛蚓%鎘%溶酶體%土壤汙染物%中性紅保留時間
삼배모인%력%용매체%토양오염물%중성홍보류시간
Pheretima asperillum%Cadmium%Lysosome%Soil pollutants%Neutral red retention time
目的 观察不同浓度镉离子诱导饲养对参环毛蚓体腔细胞溶酶体膜的毒性效应.方法 选取健康参环毛蚓70只,按照饲养土壤镉离子浓度的不同随机分为7组,每组10只.各组土壤镉离子浓度分别为0.28(对照组)、3.28、6.28、12.28、18.28、24.28和30.28 mg/kg,采用不同浓度镉离子诱导参环毛蚓染毒并饲养14 d.诱导饲养7 d和14 d时观察参环毛蚓体腔细胞的形态变化,测定其体腔细胞溶酶体中性红保留时间.结果 高浓度镉离子诱导参环毛蚓体腔细胞溶酶体的损害明显.诱导饲养7 d,对照组、3.28、6.28、12.28、18.28、24.28和30.28 mg/kg镉离子浓度组参环毛蚓体腔细胞溶酶体中性红保留时间随着镉离子浓度的增加而逐渐缩短,分别为(71.3±2.4)、(60.5±1.6)、(55.1±2.9)、(51.9±3.6)、(46.0±2.5)、(38.8±1.8)、(34.2±2.0)min.与对照组比较,诱导饲养7 d、14d时各实验组参环毛蚓溶酶体中性红保留时间明显缩短(均P＜0.05).结论 体腔细胞溶酶体中性红保留时间可用于评价土壤中重金属镉对参环毛蚓的毒性效应.
目的 觀察不同濃度鎘離子誘導飼養對參環毛蚓體腔細胞溶酶體膜的毒性效應.方法 選取健康參環毛蚓70隻,按照飼養土壤鎘離子濃度的不同隨機分為7組,每組10隻.各組土壤鎘離子濃度分彆為0.28(對照組)、3.28、6.28、12.28、18.28、24.28和30.28 mg/kg,採用不同濃度鎘離子誘導參環毛蚓染毒併飼養14 d.誘導飼養7 d和14 d時觀察參環毛蚓體腔細胞的形態變化,測定其體腔細胞溶酶體中性紅保留時間.結果 高濃度鎘離子誘導參環毛蚓體腔細胞溶酶體的損害明顯.誘導飼養7 d,對照組、3.28、6.28、12.28、18.28、24.28和30.28 mg/kg鎘離子濃度組參環毛蚓體腔細胞溶酶體中性紅保留時間隨著鎘離子濃度的增加而逐漸縮短,分彆為(71.3±2.4)、(60.5±1.6)、(55.1±2.9)、(51.9±3.6)、(46.0±2.5)、(38.8±1.8)、(34.2±2.0)min.與對照組比較,誘導飼養7 d、14d時各實驗組參環毛蚓溶酶體中性紅保留時間明顯縮短(均P＜0.05).結論 體腔細胞溶酶體中性紅保留時間可用于評價土壤中重金屬鎘對參環毛蚓的毒性效應.
목적 관찰불동농도력리자유도사양대삼배모인체강세포용매체막적독성효응.방법 선취건강삼배모인70지,안조사양토양력리자농도적불동수궤분위7조,매조10지.각조토양력리자농도분별위0.28(대조조)、3.28、6.28、12.28、18.28、24.28화30.28 mg/kg,채용불동농도력리자유도삼배모인염독병사양14 d.유도사양7 d화14 d시관찰삼배모인체강세포적형태변화,측정기체강세포용매체중성홍보류시간.결과 고농도력리자유도삼배모인체강세포용매체적손해명현.유도사양7 d,대조조、3.28、6.28、12.28、18.28、24.28화30.28 mg/kg력리자농도조삼배모인체강세포용매체중성홍보류시간수착력리자농도적증가이축점축단,분별위(71.3±2.4)、(60.5±1.6)、(55.1±2.9)、(51.9±3.6)、(46.0±2.5)、(38.8±1.8)、(34.2±2.0)min.여대조조비교,유도사양7 d、14d시각실험조삼배모인용매체중성홍보류시간명현축단(균P＜0.05).결론 체강세포용매체중성홍보류시간가용우평개토양중중금속력대삼배모인적독성효응.
Objective To investigate the toxicity effects of induced breeding with various ion concentrations of cadmium on the lysosomal membrane of coelomocytes in Pheretima aspergillum. Methods Seventy healthy Pheretima aspergilla were selected and randomized into 7 groups (n=10 each) according to different levels of cadmium ion in the soils [0.28 mg/kg (control group) , 3.28 mg/kg, 6.28 mg/kg, 12.28 mg/kg, 18.28 mg/kg, 24.28 mg/kg and 30.28 mg/kg, respectively] and were bred with the contaminating cadmium ions for 14 days. On day 7 and day 14, the morphological changes in coelomocytes were studied and neutral red retention time (NRRT) of coelomocyte lysosome was determined in Pheretima aspergilla.Results High levels of cadmium ion were associated with apparent damages to the coelomocyte lysosome in Pheretima asperillum. NRRT decreased along with increasing levels of cadmium ion by (71.3±2.4) min ,(60.5±1.6) min, (55.1±2.9) min, (51.9±3.6) min, (46.0±2.5) min, (38.8±1.8) min and (34.2±2.0) min in the groups containing 0.28 mg/kg (control group), 3.28 mg/kg, 6.28 mg/kg, 12.28 mg/kg, 18.28 mg/kg,24.28 mg/kg and 30.28 mg/kg cadmium on day 7. Compared with the control group, NRRT of other groups was significantly shorter on day 7 and day 14 (all P＜0.05). Conclusion NRRT can be used to evaluate the toxicity effects in Pheretima asperillum exposed to soils contaminated by metal cadmium.
