中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2009年
1期
41-44
,共4页
赵祝香%蒋明彦%赵子文%马为%钟维农
趙祝香%蔣明彥%趙子文%馬為%鐘維農
조축향%장명언%조자문%마위%종유농
胸腺肽α1%T淋巴细胞%细胞增殖%抗原,CD28%CD152抗原
胸腺肽α1%T淋巴細胞%細胞增殖%抗原,CD28%CD152抗原
흉선태α1%T림파세포%세포증식%항원,CD28%CD152항원
Thymosin-α1%T-lymphocytes%Cell proliferation%Antigens,CD28%CD152antigens
目的 探讨胸腺肽α1对人外周血T淋巴细胞增殖的影响及其机制.方法 采用细胞膜染料PKH26染色和荧光标记特异抗体联合流式细胞术检测胸腺肽α1对人外周血T淋巴细胞增殖及协同刺激分子表达的影响.结果 流式细胞分析显示胸腺肽α1作用5 d后,胸腺肽α1组T淋巴细胞增殖指数2.15±1.09,高于阴性对照组1.67±0.55(P=0.04);胸腺肽α1组协同刺激分子CD28、CD152的表达率均高于阴性对照组,但只有CD28的表达率两组差异有统计学意义[CD28:(27.63±12.36)%比(22.62±10.12)%,P=0.04;CD152:(3.54±1.58)%比(0.91±0.41)%,P=0.46].结论 胸腺肽α1可明显促进人外周血T淋巴细胞增殖,其机制可能与上调协同刺激分子CD28的表达有关.
目的 探討胸腺肽α1對人外週血T淋巴細胞增殖的影響及其機製.方法 採用細胞膜染料PKH26染色和熒光標記特異抗體聯閤流式細胞術檢測胸腺肽α1對人外週血T淋巴細胞增殖及協同刺激分子錶達的影響.結果 流式細胞分析顯示胸腺肽α1作用5 d後,胸腺肽α1組T淋巴細胞增殖指數2.15±1.09,高于陰性對照組1.67±0.55(P=0.04);胸腺肽α1組協同刺激分子CD28、CD152的錶達率均高于陰性對照組,但隻有CD28的錶達率兩組差異有統計學意義[CD28:(27.63±12.36)%比(22.62±10.12)%,P=0.04;CD152:(3.54±1.58)%比(0.91±0.41)%,P=0.46].結論 胸腺肽α1可明顯促進人外週血T淋巴細胞增殖,其機製可能與上調協同刺激分子CD28的錶達有關.
목적 탐토흉선태α1대인외주혈T림파세포증식적영향급기궤제.방법 채용세포막염료PKH26염색화형광표기특이항체연합류식세포술검측흉선태α1대인외주혈T림파세포증식급협동자격분자표체적영향.결과 류식세포분석현시흉선태α1작용5 d후,흉선태α1조T림파세포증식지수2.15±1.09,고우음성대조조1.67±0.55(P=0.04);흉선태α1조협동자격분자CD28、CD152적표체솔균고우음성대조조,단지유CD28적표체솔량조차이유통계학의의[CD28:(27.63±12.36)%비(22.62±10.12)%,P=0.04;CD152:(3.54±1.58)%비(0.91±0.41)%,P=0.46].결론 흉선태α1가명현촉진인외주혈T림파세포증식,기궤제가능여상조협동자격분자CD28적표체유관.
Objective To elucidate the effects of thymosin- α 1 on the proliferation of human peripheral blood T lymphocytes and the mechanisms. Methods Membrane dyes PKH26 stain and fluorescent special antibody combined with flow cytometry were used to evaluate the effects of thymosin-α1 on human peripheral blood T lymphocytes proliferation and the expression of co- stimulatory molecules. Results After stimulation with thymosin- α 1 for 5 days, flow cytometric analysis showed that the proliferation index of thymosin- α1 group was significantly higher than that of control group (2.15±1.09 vs 1.67±0.55, P= 0.04). The percentage of positive co-stimulatory molecule CD28 in the thymosinα1 group was significantly higher than that in the control group [(27.63±12.36)% vs (22.62±10.12)%, P= 0.04)]. But there was no difference in the percentage of co-stimulatory molecule CD152 between thymosin-α1 group and control group [(3.54±1.58)% vs (0.91±0.41)%, P= 0.46]. Conclusion In vitro thymosin-α1 can obviously promote human peripheral blood T lymphocytes proliferation, and its mechanism may be related to up-regulation on expression level of co-stimulatory molecule CD28 by thymosin-α1.
