中国医师进修杂志
中國醫師進脩雜誌
중국의사진수잡지
CHINESE JOURNAL OF POSTGRADUATES OF MEDICINE
2011年
29期
1-4
,共4页
胡传义%刘修恒%葛名欢%宋超
鬍傳義%劉脩恆%葛名歡%宋超
호전의%류수항%갈명환%송초
肾肿瘤%新生血管化,病理性%肝细胞生长因子激活物抑制因子-1
腎腫瘤%新生血管化,病理性%肝細胞生長因子激活物抑製因子-1
신종류%신생혈관화,병이성%간세포생장인자격활물억제인자-1
Kidney neoplasms%Neovascularization,pathologic%Hepatocyte growth factor activator inhibitor-1
目的 探讨肝细胞生长因子激活物抑制因子-1(HAI-1)基因在肾癌组织中的表达及其与肿瘤血管生成的关系.方法 应用半定量反转录-聚合酶链反应(RT-PCR)法检测HAI-1基因在56例肾癌组织和相应癌旁非癌肾组织及12例正常肾组织中的表达水平；采用免疫组织化学SP法测定上述组织中CD34的表达,并测定微血管密度(MVD),分析HAI-1基因的表达与肾癌临床病理特征及MVD的关系.结果 HAI-1基因在肾癌组织、癌旁非癌肾组织、正常肾组织中的阳性率分别为58.93％ (33/56)、76.79％ (43/56)和91.67％(11/12),肾癌组织中HAI-1基因阳性率显著低于癌旁非癌肾组织和正常肾组织(P＜ 0.05)；HAI-1基因的表达与Fuhrman病理分级、Robson临床分期呈负相关(r＝ -0.617、-0.432,P＜0.01或＜0.05)；肾癌组织有淋巴结转移者的HAI-1基因阳性率[35.29％(6/17)]显著低于无淋巴结转移者[69.23％( 27/39)],差异有统计学意义(P＜0.05).肾癌组织中MVD显著高于癌旁非癌肾组织和正常肾组织,差异有统计学意义(P＜0.05)；肾癌组织中HAI-1基因的表达与MVD呈负相关(r＝-0.516,P＜0.01).结论 HAI-1基因表达缺失与肾癌的发生和发展、浸润和转移存在一定的关系,HAI-1基因表达缺失可作为肾癌浸润、转移和预后的一种重要分子标志.
目的 探討肝細胞生長因子激活物抑製因子-1(HAI-1)基因在腎癌組織中的錶達及其與腫瘤血管生成的關繫.方法 應用半定量反轉錄-聚閤酶鏈反應(RT-PCR)法檢測HAI-1基因在56例腎癌組織和相應癌徬非癌腎組織及12例正常腎組織中的錶達水平；採用免疫組織化學SP法測定上述組織中CD34的錶達,併測定微血管密度(MVD),分析HAI-1基因的錶達與腎癌臨床病理特徵及MVD的關繫.結果 HAI-1基因在腎癌組織、癌徬非癌腎組織、正常腎組織中的暘性率分彆為58.93％ (33/56)、76.79％ (43/56)和91.67％(11/12),腎癌組織中HAI-1基因暘性率顯著低于癌徬非癌腎組織和正常腎組織(P＜ 0.05)；HAI-1基因的錶達與Fuhrman病理分級、Robson臨床分期呈負相關(r＝ -0.617、-0.432,P＜0.01或＜0.05)；腎癌組織有淋巴結轉移者的HAI-1基因暘性率[35.29％(6/17)]顯著低于無淋巴結轉移者[69.23％( 27/39)],差異有統計學意義(P＜0.05).腎癌組織中MVD顯著高于癌徬非癌腎組織和正常腎組織,差異有統計學意義(P＜0.05)；腎癌組織中HAI-1基因的錶達與MVD呈負相關(r＝-0.516,P＜0.01).結論 HAI-1基因錶達缺失與腎癌的髮生和髮展、浸潤和轉移存在一定的關繫,HAI-1基因錶達缺失可作為腎癌浸潤、轉移和預後的一種重要分子標誌.
목적 탐토간세포생장인자격활물억제인자-1(HAI-1)기인재신암조직중적표체급기여종류혈관생성적관계.방법 응용반정량반전록-취합매련반응(RT-PCR)법검측HAI-1기인재56례신암조직화상응암방비암신조직급12례정상신조직중적표체수평；채용면역조직화학SP법측정상술조직중CD34적표체,병측정미혈관밀도(MVD),분석HAI-1기인적표체여신암림상병리특정급MVD적관계.결과 HAI-1기인재신암조직、암방비암신조직、정상신조직중적양성솔분별위58.93％ (33/56)、76.79％ (43/56)화91.67％(11/12),신암조직중HAI-1기인양성솔현저저우암방비암신조직화정상신조직(P＜ 0.05)；HAI-1기인적표체여Fuhrman병리분급、Robson림상분기정부상관(r＝ -0.617、-0.432,P＜0.01혹＜0.05)；신암조직유림파결전이자적HAI-1기인양성솔[35.29％(6/17)]현저저우무림파결전이자[69.23％( 27/39)],차이유통계학의의(P＜0.05).신암조직중MVD현저고우암방비암신조직화정상신조직,차이유통계학의의(P＜0.05)；신암조직중HAI-1기인적표체여MVD정부상관(r＝-0.516,P＜0.01).결론 HAI-1기인표체결실여신암적발생화발전、침윤화전이존재일정적관계,HAI-1기인표체결실가작위신암침윤、전이화예후적일충중요분자표지.
Objective To investigate the expression of hepatocyte growth factor activator inhibitor- 1 (HAl-1) gene in the tissues of renal cell carcinoma (RCC) and the correlation with tumor angiogenesis.Methods The expression of HAI-1 gene was detected by semi-quantitative RT-PCR in the tissues of 56 cases of RCC,corresponding adjacent non-cancer tissues and 12 nomad renal tissues,the expression of CD34was detected by immunohistochemistry SP method in the above-mentioned organizations,and counted the microvessel density (MVD),analyzed the relationship between the expression of HAI-1 gene in RCC and clinicopathological parameters,MVD.Results The positive expression rate of HAI-1 gene in RCC tissues,the adjacent non-cancer tissues and normal renal tissues were 58.93％(33/56),76.79％(43/56) and 91.67％(11/12),respectively.The expression of HAI-1 gene was a negative correlation to the Fuhrman histological grade,Robson clinical stage (r ＝ -0.617,-0.432,P ＜ 0.01 or ＜ 0.05 ).The positive expression rate in lymph node metastasis was significantly lower than that in without lymph node metastasis [ 35.29％(6/17) vs.69.23％(27/39),P＜ 0.05 ].The MVD in RCC tissues was significantly higher than that in the adjacent noncancer tissues and normal renal tissues(P＜ 0.05).The expression of HAI-I gene in RCC tissues was negative correlated to the MVD (r ＝ -0.516,P ＜ 0.01 ).Conclusions There is a certain relationship between the missing expression of HAI-1 gene in RCC and the development,invasion and metastasis of RCC.The HAI-1 gene deletion can be used as an important molecular marker in the invasion and metastasis of RCC.
