中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2008年
48期
3436-3439
,共4页
乳杆菌属%T淋巴细胞%B淋巴细胞%干扰素Ⅱ型%模型,免疫学
乳桿菌屬%T淋巴細胞%B淋巴細胞%榦擾素Ⅱ型%模型,免疫學
유간균속%T림파세포%B림파세포%간우소Ⅱ형%모형,면역학
Lactobacillus%T-lymphocytes%B-lymphocytes%Interferon type Ⅱ%Models,immunological
目的 探讨乳酸杆菌(LGG)对空肠弯曲菌(CJ)致敏的自身免疫病模型小鼠(CJ小鼠)免疫功能的影响.方法 ICR小鼠随机分为正常对照组、模型组、LGG组及氢化可的松(HC)组,除正常组外,其余组制备自身免疫病小鼠模型.从造模第15天起分别腹腔(或皮下)注射,连续用药7 d,造模第25大处死小鼠.用酶联免疫吸附试验(ELISA)法测定小鼠血清中ds-DNA抗体水平及脾淋巴细胞的γ干扰素(IFN-γ)分泌活性;检测脾T、B淋巴细胞的增殖活性;镜下观察肝、肾组织的病理变化.结果 LGG对ds-DNA抗体没有影响,LGG可改善脏器组织的病理性改变.LGG组GJ小鼠的T、B淋巴细胞增殖活性及脾淋巴细胞分泌IFN-γ活性明显低于模型组[0.42±0.05 vs 0.60±0.09,0.43±0.05 vs 0.60±0.09,(184v16)VS(195±6)ps/ml,均P＜0.01].结论 LGG对CJ小鼠的免疫机能有一定的免疫抑制作用.
目的 探討乳痠桿菌(LGG)對空腸彎麯菌(CJ)緻敏的自身免疫病模型小鼠(CJ小鼠)免疫功能的影響.方法 ICR小鼠隨機分為正常對照組、模型組、LGG組及氫化可的鬆(HC)組,除正常組外,其餘組製備自身免疫病小鼠模型.從造模第15天起分彆腹腔(或皮下)註射,連續用藥7 d,造模第25大處死小鼠.用酶聯免疫吸附試驗(ELISA)法測定小鼠血清中ds-DNA抗體水平及脾淋巴細胞的γ榦擾素(IFN-γ)分泌活性;檢測脾T、B淋巴細胞的增殖活性;鏡下觀察肝、腎組織的病理變化.結果 LGG對ds-DNA抗體沒有影響,LGG可改善髒器組織的病理性改變.LGG組GJ小鼠的T、B淋巴細胞增殖活性及脾淋巴細胞分泌IFN-γ活性明顯低于模型組[0.42±0.05 vs 0.60±0.09,0.43±0.05 vs 0.60±0.09,(184v16)VS(195±6)ps/ml,均P＜0.01].結論 LGG對CJ小鼠的免疫機能有一定的免疫抑製作用.
목적 탐토유산간균(LGG)대공장만곡균(CJ)치민적자신면역병모형소서(CJ소서)면역공능적영향.방법 ICR소서수궤분위정상대조조、모형조、LGG조급경화가적송(HC)조,제정상조외,기여조제비자신면역병소서모형.종조모제15천기분별복강(혹피하)주사,련속용약7 d,조모제25대처사소서.용매련면역흡부시험(ELISA)법측정소서혈청중ds-DNA항체수평급비림파세포적γ간우소(IFN-γ)분비활성;검측비T、B림파세포적증식활성;경하관찰간、신조직적병리변화.결과 LGG대ds-DNA항체몰유영향,LGG가개선장기조직적병이성개변.LGG조GJ소서적T、B림파세포증식활성급비림파세포분비IFN-γ활성명현저우모형조[0.42±0.05 vs 0.60±0.09,0.43±0.05 vs 0.60±0.09,(184v16)VS(195±6)ps/ml,균P＜0.01].결론 LGG대CJ소서적면역궤능유일정적면역억제작용.
Objective To investigate the immune effects of Lactobacillus rhamnosus GG (LGG) on immune response.Methods Fourty 1CR mice were randomly divided into 4 groups, normal control (N) group, model (M) group, LactobaciUus (LGG) group, and hydrocortisone (HC) group.The mice were induced by Campylobacter jejuni with complete Freund's adjuvant as autoimmune animal model except N group.From the fifteenth day after primary immunization, each group was given different administration through peritoneum route for 7 days.Twenty-five days after the model establishment, the mice were killed.Then the blood sample was collected the undergo ELISA to detect the level of ds-DNA antibody and interferon (IFN)-γ.The proliferation activities of the B and T cells were examined.The livers and kidneys were taken out to undergo microscopy.Results The ds-DNA antibody levels of the HC and LGG groups were not significant different from that of the M group (both P 0.05).The proliferation activities of T and B cells and IFN-γlevel of the groups LGG were all significantly lower than those of the group M[0.42 ±0.05 vs 0.60 ±0.09,0.43 ± 0.05 vs 0.60 ± 0.09, (184 ± 16) vs (195±6) pg/ml, all P < 0.01].Conclusion LGG inhibits the immune response induced by CJ to a eertain degree.
