中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2010年
7期
914-916
,共3页
张善义%李军亮%许新科%郑眉光%温铖彩%李方成
張善義%李軍亮%許新科%鄭眉光%溫鋮綵%李方成
장선의%리군량%허신과%정미광%온성채%리방성
胶质瘤%光动力治疗%血卟啉单甲醚
膠質瘤%光動力治療%血卟啉單甲醚
효질류%광동력치료%혈계람단갑미
Glioma%Photodynamic therapy%Hematoporphyrin monomethyl ether
目的 观察光动力学疗法(PDT)对人胶质瘤细胞株U87-MG抗原加工相关转运体1(TAP1)表达的影响.方法 CCK-8法确立光敏剂最适浓度.实验随机分为5组:A、B、C、D组细胞与光敏剂避光孵育2h后,接受激光照射,能量密度分别0、1.2,2.4A.2J/cm2;E组未予处理(无光敏剂、无激光).PDT后2、12 h分别收集细胞,实时定量聚合酶链反应(realtime-PCR)检测;12、24 h提取蛋白,进行Western blot分析.结果 处理后TAP1基因转录水平明显升高:2h时,1.2、2.4A.2 J/cm2组分别上调11.070、5.925、4.175倍;12h时,分别上调10.400、4.560、4.023倍.TAP1蛋白在PDT后12 h,0、1.2、2.4、4.2 J/cm2组分别上调1.17、2.65、1.92、1.37倍;24 h,分别上调1.13、2.43、2.38、2.09倍,差异有统计学意义(P<0.01).结论 PDT上调TAP1基因的转录和表达,并呈剂量依赖性和时间依赖性,从而增强肿瘤细胞抗原处理及提呈能力.
目的 觀察光動力學療法(PDT)對人膠質瘤細胞株U87-MG抗原加工相關轉運體1(TAP1)錶達的影響.方法 CCK-8法確立光敏劑最適濃度.實驗隨機分為5組:A、B、C、D組細胞與光敏劑避光孵育2h後,接受激光照射,能量密度分彆0、1.2,2.4A.2J/cm2;E組未予處理(無光敏劑、無激光).PDT後2、12 h分彆收集細胞,實時定量聚閤酶鏈反應(realtime-PCR)檢測;12、24 h提取蛋白,進行Western blot分析.結果 處理後TAP1基因轉錄水平明顯升高:2h時,1.2、2.4A.2 J/cm2組分彆上調11.070、5.925、4.175倍;12h時,分彆上調10.400、4.560、4.023倍.TAP1蛋白在PDT後12 h,0、1.2、2.4、4.2 J/cm2組分彆上調1.17、2.65、1.92、1.37倍;24 h,分彆上調1.13、2.43、2.38、2.09倍,差異有統計學意義(P<0.01).結論 PDT上調TAP1基因的轉錄和錶達,併呈劑量依賴性和時間依賴性,從而增彊腫瘤細胞抗原處理及提呈能力.
목적 관찰광동역학요법(PDT)대인효질류세포주U87-MG항원가공상관전운체1(TAP1)표체적영향.방법 CCK-8법학립광민제최괄농도.실험수궤분위5조:A、B、C、D조세포여광민제피광부육2h후,접수격광조사,능량밀도분별0、1.2,2.4A.2J/cm2;E조미여처리(무광민제、무격광).PDT후2、12 h분별수집세포,실시정량취합매련반응(realtime-PCR)검측;12、24 h제취단백,진행Western blot분석.결과 처리후TAP1기인전록수평명현승고:2h시,1.2、2.4A.2 J/cm2조분별상조11.070、5.925、4.175배;12h시,분별상조10.400、4.560、4.023배.TAP1단백재PDT후12 h,0、1.2、2.4、4.2 J/cm2조분별상조1.17、2.65、1.92、1.37배;24 h,분별상조1.13、2.43、2.38、2.09배,차이유통계학의의(P<0.01).결론 PDT상조TAP1기인적전록화표체,병정제량의뢰성화시간의뢰성,종이증강종류세포항원처리급제정능력.
Objective To investigate the effect of hematoporphyrin monomethyl ether (HMME)-based photodynamic treatment on gene expression of transporter associated with antigen processing 1 (TAP1) in human U87-MG glioma. Methods Cells were incubated with 10 g/L HMME for 2 h and irradiated with fluence of 0, 1.2, 2.4, 4. 2 J/cm2. RNA was isolated at 2 and 12 h post-treatment and from one control (untreated) , and the expression changes were observed by real-time reverse transcription-poly-merase chain reaction ( RT-PCR). Protein was isolated at 12 and 24 h post-treatment and from one control, and the expression level was detected by Western blotting. Results Post-treatment, the expression of TAP1 mRNA and protein was obviously increased as compared with the control group (all P<0.01). Conclusion The therapeutics of HMME-mediated PDT might have more pronounced effect on TAP1 mRNA and protein expression, indicating that the effect may be involved in the antigen processing and transporting and that PDT activated immune response and cytotoxic T lymphocytes and contributed to the immu-nological recognition.
