CAJ | 학술논문

目的探讨建立简捷高效原代小胶质细胞纯化培养方法 .方法以小胶质细胞原代培养McCarthy经典培养方法为基础,分为常规对照组、机械分离组、胰酶消化组和利多卡因组共四组培养,并对比观察记录形态变化并计数,借助CD68及OX42抗体间接免疫荧光标记进行鉴定、计算纯度.结果三种分离方法均获得了较高纯度和产量的小胶质细胞,以利多卡因组为佳,纯度为(94.92±7.05)%,流式细胞结果显示细胞纯度约为(96.2±2.8)%.结论小胶质原代细胞培养过程中,采用利多卡因处理可显著提高细胞分离效率及纯度.
목적 탐토건립간첩고효원대소효질세포순화배양방법 .방법 이소효질세포원대배양McCarthy경전배양방법 위기출,분위상규대조조、궤계분리조、이매소화조화리다잡인조공사조배양,병대비관찰기록형태변화병계수,차조CD68급OX42항체간접면역형광표기진행감정、계산순도.결과 삼충분리방법 균획득료교고순도화산량적소효질세포,이리다잡인조위가,순도위(94.92±7.05)%,류식세포결과 현시세포순도약위(96.2±2.8)%.결론 소효질원대세포배양과정중,채용리다잡인처리가현저제고세포분리효솔급순도.
Objective To find an easy and effective method for primary culture and pttrification of microgha cells.Methods Based on classical microgha primary culture method of McCarthy,four groups (control group,mechanical separation group,enzyme digestion group and lidocaine separation group)were cultured.Morphologic changes and cell count were recorded and compared.Identification and appraisement of microglia cells were under the assistance of immunofluoreseence mediated by CD68 and OX42 antibodies.Results All groups got high purity and quantity of mieroglia cells,The highest purity was obtained in lidocaine separation group[(94.92±7.05)%].The purity of mieroglia cells was(96.2±2.8)% determined by flow cytometry.Conclusion In the primary culture of microgha cells,using lidoeaine separation can elevate microglia cell separation efficiency and the purity significandy.