中华血液学杂志
中華血液學雜誌
중화혈액학잡지
Chinese Journal of Hematology
2001年
1期
27-29
,共3页
王莉%董陆佳%刘广贤%林育松%张婧%贾蜀琼%卢淑娟%骈淮媛%姚波%陈虎
王莉%董陸佳%劉廣賢%林育鬆%張婧%賈蜀瓊%盧淑娟%駢淮媛%姚波%陳虎
왕리%동륙가%류엄현%림육송%장청%가촉경%로숙연%병회원%요파%진호
白血病%基因,GATA-2%聚合酶链反应
白血病%基因,GATA-2%聚閤酶鏈反應
백혈병%기인,GATA-2%취합매련반응
目的 探讨白血病患者GATA-2基因的表达及其临床意义。方法 应用RT-PCR对各类白血病患者GATA-2的表达进行检测，慢性粒细胞白血病（CML）和急性早幼粒细胞白血病（APL）同时检测bcr/abl和PML/RARα的表达。结果 初治/复发的急性髓系白血病（AML）和急性淋巴细胞白血病（ALL）GATA-2的阳性率分别为93%和70%，慢性粒细胞白血病慢性期（CML-CP）为83%。正常人骨髓和外周血未检测到GATA-2。缓解期AML患者GATA-2的表达与初治/复发AML相比，差异无显著性；移植治疗后患者GATA-2的表达率显著下降；移植治疗后的CML患者在bcr/abl转阴后仍可检出GATA-2，而移植治疗后APL患者PML/RARα和GATA-2都为阴性。结论 GATA-2基因转录产物在正常人骨髓和外周血中低表达，在各类白血病中高表达。缓解期骨髓中仍有GATA-2的高表达，移植治疗后GATA-2表达显著下降。GATA-2高表达反映了患者体内残存的白血病干细胞，自体或异基因骨髓移植可以更好地清除白血病干细胞。
目的 探討白血病患者GATA-2基因的錶達及其臨床意義。方法 應用RT-PCR對各類白血病患者GATA-2的錶達進行檢測，慢性粒細胞白血病（CML）和急性早幼粒細胞白血病（APL）同時檢測bcr/abl和PML/RARα的錶達。結果 初治/複髮的急性髓繫白血病（AML）和急性淋巴細胞白血病（ALL）GATA-2的暘性率分彆為93%和70%，慢性粒細胞白血病慢性期（CML-CP）為83%。正常人骨髓和外週血未檢測到GATA-2。緩解期AML患者GATA-2的錶達與初治/複髮AML相比，差異無顯著性；移植治療後患者GATA-2的錶達率顯著下降；移植治療後的CML患者在bcr/abl轉陰後仍可檢齣GATA-2，而移植治療後APL患者PML/RARα和GATA-2都為陰性。結論 GATA-2基因轉錄產物在正常人骨髓和外週血中低錶達，在各類白血病中高錶達。緩解期骨髓中仍有GATA-2的高錶達，移植治療後GATA-2錶達顯著下降。GATA-2高錶達反映瞭患者體內殘存的白血病榦細胞，自體或異基因骨髓移植可以更好地清除白血病榦細胞。
목적 탐토백혈병환자GATA-2기인적표체급기림상의의。방법 응용RT-PCR대각류백혈병환자GATA-2적표체진행검측，만성립세포백혈병（CML）화급성조유립세포백혈병（APL）동시검측bcr/abl화PML/RARα적표체。결과 초치/복발적급성수계백혈병（AML）화급성림파세포백혈병（ALL）GATA-2적양성솔분별위93%화70%，만성립세포백혈병만성기（CML-CP）위83%。정상인골수화외주혈미검측도GATA-2。완해기AML환자GATA-2적표체여초치/복발AML상비，차이무현저성；이식치료후환자GATA-2적표체솔현저하강；이식치료후적CML환자재bcr/abl전음후잉가검출GATA-2，이이식치료후APL환자PML/RARα화GATA-2도위음성。결론 GATA-2기인전록산물재정상인골수화외주혈중저표체，재각류백혈병중고표체。완해기골수중잉유GATA-2적고표체，이식치료후GATA-2표체현저하강。GATA-2고표체반영료환자체내잔존적백혈병간세포，자체혹이기인골수이식가이경호지청제백혈병간세포。
Objective To investigate GATA-2 gene expression in leukemiapatients and its clinical significance. Methods GATA-2 gene transcripts were detected by reverse transcriptase polymerase chain reaction (RT-PCR), and bcr/abl or PML/RAR α transcripts were detected in patients with chronic myeloid leukemia (CML) and acute promyelocytic leukemia (APL), respectively. Results The GATA-2 transcripts were revealed in 93% of acute myeloid leukemia (AML), in 70% of acute lymphoblastic leukemia (ALL), and 83% of CML patients, but not in normal bone marrow and peripheral blood cells. GATA-2 expression in remission patients was similar to newly diagnosed or relapsed patients. In post-transplantation patients, GATA-2 expression was significantly decreased. In these cases GATA-2 transcripts could be detected with bcr/abl fusion gene disappeared in 2 of 12 CML, whereas neither GATA-2 nor PML/RARα transcripts could be detected in APL. Conclusion GATA-2 transcripts was highly expressed in leukemia patients and had no change in remission but significantly decreased in post-transplantation patients. Detection of GATA-2 expression would give information about the residual leukemia stem cells.
