中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2011年
2期
123-126
,共4页
羟乙基淀粉%重症急性胰腺炎%水通道蛋白-1%毛细血管渗漏%肝损伤
羥乙基澱粉%重癥急性胰腺炎%水通道蛋白-1%毛細血管滲漏%肝損傷
간을기정분%중증급성이선염%수통도단백-1%모세혈관삼루%간손상
Hydroxyethyl starch%Pancreatitis,severe acute%Liver injury%Aquaporin-1%Capillary Permeability
目的 研究羟乙基淀粉130/0.4(万汶)对重症急性胰腺炎大鼠肝脏水通道蛋白-1(AQP-1)表达及肝脏损伤的影响.方法 将雄性SD大鼠48只按完全随机法分为假手术组(sham operation group,Sham组)、重症急性胰腺炎组(severe acute pancreatitis,SAP组)、羟乙基淀粉治疗组(HES组),每组分6 h、24 h两个时间点,每个时间点8只.以5%牛磺脱氧胆酸钠逆行胰胆管注射建立SAP模型.各组于造模后6 h、24 h处死大鼠,检测血淀粉酶(AMY),谷丙转氨酶(ALT),谷草转氨酶(AST),肝脏含水量,HE染色观察胰腺、肝脏组织病理,酶联免疫吸附(ELISA)方法检测血清TNF-α水平,RT-PCR检测肝组织AQP-1mRNA,免疫组化检测肝组织AQP-1蛋白.结果 各时间点SAP组与假手术组比较,血淀粉酶、ALT、AST、肝脏含水量、血清TNF-α水平、AQP-1mRNA及AQP-1蛋白均显著上调(P＜0.05);HES组与SAP组比较,血淀粉酶、ALT、AST、肝脏含水量、血清TNF-α水平、AQP-1mRNA及AQP-1蛋白均显著下调(P＜0.05).6 h时间点,Sham组、SAP组和HES组AQP-1mRNA表达量分别为(0.402±0.023)mmol/L、(0.811±0.032)mmol/L和(0.595±0.015)mmol/L,3组间差异有统计学意义(P＜0.05);24 h时间点,各组AQP-1mRNA表达量分别为(0.412±0.017)mmol/L、(0.823±0.029)mmol/L和(0.607±0.021)mmol/L,3组间差异有统计学意义(P＜0.05).6 h时间点,Sham组、SAP组和HES组AQP-1蛋白表达分别为(2.07±0.25)、(6.90±0.38)和(4.48±0.29),3组间差异有统计学意义(P＜0.05);24 h时间点,各组AQP-1蛋白表达分别为(2.32±0.31)、(7.04±0.32)和(4.56±0.25),3组间差异有统计学意义(P＜0.05).胰腺、肝脏组织病理HES组较SAP组明显改善.结论 HES130/0.4可一定程度改善重症急性胰腺炎的肝脏损伤,减轻毛细血管渗漏,AQP-1在重症急性胰腺炎肝脏损伤导致的毛细血管渗漏中起一定作用.
目的 研究羥乙基澱粉130/0.4(萬汶)對重癥急性胰腺炎大鼠肝髒水通道蛋白-1(AQP-1)錶達及肝髒損傷的影響.方法 將雄性SD大鼠48隻按完全隨機法分為假手術組(sham operation group,Sham組)、重癥急性胰腺炎組(severe acute pancreatitis,SAP組)、羥乙基澱粉治療組(HES組),每組分6 h、24 h兩箇時間點,每箇時間點8隻.以5%牛磺脫氧膽痠鈉逆行胰膽管註射建立SAP模型.各組于造模後6 h、24 h處死大鼠,檢測血澱粉酶(AMY),穀丙轉氨酶(ALT),穀草轉氨酶(AST),肝髒含水量,HE染色觀察胰腺、肝髒組織病理,酶聯免疫吸附(ELISA)方法檢測血清TNF-α水平,RT-PCR檢測肝組織AQP-1mRNA,免疫組化檢測肝組織AQP-1蛋白.結果 各時間點SAP組與假手術組比較,血澱粉酶、ALT、AST、肝髒含水量、血清TNF-α水平、AQP-1mRNA及AQP-1蛋白均顯著上調(P＜0.05);HES組與SAP組比較,血澱粉酶、ALT、AST、肝髒含水量、血清TNF-α水平、AQP-1mRNA及AQP-1蛋白均顯著下調(P＜0.05).6 h時間點,Sham組、SAP組和HES組AQP-1mRNA錶達量分彆為(0.402±0.023)mmol/L、(0.811±0.032)mmol/L和(0.595±0.015)mmol/L,3組間差異有統計學意義(P＜0.05);24 h時間點,各組AQP-1mRNA錶達量分彆為(0.412±0.017)mmol/L、(0.823±0.029)mmol/L和(0.607±0.021)mmol/L,3組間差異有統計學意義(P＜0.05).6 h時間點,Sham組、SAP組和HES組AQP-1蛋白錶達分彆為(2.07±0.25)、(6.90±0.38)和(4.48±0.29),3組間差異有統計學意義(P＜0.05);24 h時間點,各組AQP-1蛋白錶達分彆為(2.32±0.31)、(7.04±0.32)和(4.56±0.25),3組間差異有統計學意義(P＜0.05).胰腺、肝髒組織病理HES組較SAP組明顯改善.結論 HES130/0.4可一定程度改善重癥急性胰腺炎的肝髒損傷,減輕毛細血管滲漏,AQP-1在重癥急性胰腺炎肝髒損傷導緻的毛細血管滲漏中起一定作用.
목적 연구간을기정분130/0.4(만문)대중증급성이선염대서간장수통도단백-1(AQP-1)표체급간장손상적영향.방법 장웅성SD대서48지안완전수궤법분위가수술조(sham operation group,Sham조)、중증급성이선염조(severe acute pancreatitis,SAP조)、간을기정분치료조(HES조),매조분6 h、24 h량개시간점,매개시간점8지.이5%우광탈양담산납역행이담관주사건립SAP모형.각조우조모후6 h、24 h처사대서,검측혈정분매(AMY),곡병전안매(ALT),곡초전안매(AST),간장함수량,HE염색관찰이선、간장조직병리,매련면역흡부(ELISA)방법검측혈청TNF-α수평,RT-PCR검측간조직AQP-1mRNA,면역조화검측간조직AQP-1단백.결과 각시간점SAP조여가수술조비교,혈정분매、ALT、AST、간장함수량、혈청TNF-α수평、AQP-1mRNA급AQP-1단백균현저상조(P＜0.05);HES조여SAP조비교,혈정분매、ALT、AST、간장함수량、혈청TNF-α수평、AQP-1mRNA급AQP-1단백균현저하조(P＜0.05).6 h시간점,Sham조、SAP조화HES조AQP-1mRNA표체량분별위(0.402±0.023)mmol/L、(0.811±0.032)mmol/L화(0.595±0.015)mmol/L,3조간차이유통계학의의(P＜0.05);24 h시간점,각조AQP-1mRNA표체량분별위(0.412±0.017)mmol/L、(0.823±0.029)mmol/L화(0.607±0.021)mmol/L,3조간차이유통계학의의(P＜0.05).6 h시간점,Sham조、SAP조화HES조AQP-1단백표체분별위(2.07±0.25)、(6.90±0.38)화(4.48±0.29),3조간차이유통계학의의(P＜0.05);24 h시간점,각조AQP-1단백표체분별위(2.32±0.31)、(7.04±0.32)화(4.56±0.25),3조간차이유통계학의의(P＜0.05).이선、간장조직병리HES조교SAP조명현개선.결론 HES130/0.4가일정정도개선중증급성이선염적간장손상,감경모세혈관삼루,AQP-1재중증급성이선염간장손상도치적모세혈관삼루중기일정작용.
Objective To investigate the effect of hydroxyethyl starch (HES 130/0. 4) on the expression of aquaporin 1 (AQP-1) and liver injury in rats with severe acute pancreatitis(SAP).Methods Forty-eight male SD rats were randomly divided into three groups: Sham, SAP, and HES;each group was divided into 6 hour and 24 hour timepoints, with 8 in each subgroup. An SAP model was induced by injecting 5% sodium taurodeoxycholate into the biliary pancreatic duct. AMY, ALT,AST and water content in the liver were measured and TNF-a was examined by ELISA, and the expression of liver AQP-1mRNA was determined by RT-PCR, and the expression of liver AQP-Ⅰ protein was evaluated by immunohistochemical methods. Results Compared with the Sham group, the level of AMY, ALT, AST, TNF-a, water content, AQP-1mRNA and AQP-1 protein increased significantly in the SAP group (P＜0.05). Compared with the SAP group, the level of AMY, ALT, AST,TNF-a, water content, AQP-1mRNA and AQP-1 protein decreased significantly in the HES group (P＜0.05). At 6 hours, the expressions of liver AQP-1mRNA were (0. 402 ± 0. 023), (0. 811 ±0. 032) and (0. 595 ± 0. 015) in the Sham, SAP, and HES groups, respectively; at 24 hours, they were(0. 412 ± 0. 017), ( 0. 823 ± 0. 029) and (0. 607 ± 0. 021 ), with significant differences between each group (P＜0. 05). At 6 hours, the expressions of liver AQP-1 proteins were (2.07±0.25),(6.90±0.38)and (4.48±0.29) in the Sham, SAP and HES groups, respectively;at 24 hours, they were (2. 32±0. 31 ), (7. 04 ± 0. 32) and (4. 56 ± 0. 35), with significant differences between each group (P＜0. 05). Compared with the SAP group, the pathology of the pancreas and liver ameliorated significantly in the HES group. Conclusions Hydroxyethyl starch 130/0.4 may ameliorate liver injury of severe acute pancreatitis and alleviate the capillary leak. AQP-1 may play a role in the capillary leak caused by the liver injury of severe acute pancreatitis.
