CAJ | 학술논문

目的通过时小鼠阴道白色假丝酵母菌病感染过程中受激活调节正常T细胞表达和分泌因子(RANTESF)的变化,探讨其在阴道白色假丝酵母菌病局部免疫中的作用.结果将雌性63只8～10周龄小鼠分为3组,分别为对照组、一次感染组,二次感染组,通过小鼠阴道种植白色假丝酵母菌制做小鼠阴道白色假丝酵母菌感染模型,分别于2、7、14 d取小鼠阴道灌洗液及血液测RANTESF因子的含量,并留取部分阴道灌洗液做真菌培养.结果第一次感染组及第二次感染组血液及191道灌洗液中的RAN-TESF因子的含量在感染第7天达到高峰,感染第2天及第14天含量无显著变化,含量均高于对照组.第二次感染组同时期RAN.TESF因子含量较第一次感染组显著增多.第一次感染组及第二次感染组同时期阴道灌洗液中RANTESF因子含量较血液中显著增多.阴道灌洗液真菌培养显示第一次、第二次感染组cfu计数于第7天达到高峰,第14天与第2天无显著差异.第二次感染组同时期真菌培养cfu计数比第一次感染组显著增多.结论 RANTESF因子在抗白色假丝酵母菌的感染过程中发挥了作用,主要是通过阴道局部黏膜免疫发挥作用.且在再次感染过程中发挥较大作用.
목적 통과시소서음도백색가사효모균병감염과정중수격활조절정상T세포표체화분비인자(RANTESF)적변화,탐토기재음도백색가사효모균병국부면역중적작용.결과 장자성63지8～10주령소서분위3조,분별위대조조、일차감염조,이차감염조,통과소서음도충식백색가사효모균제주소서음도백색가사효모균감염모형,분별우2、7、14 d취소서음도관세액급혈액측RANTESF인자적함량,병류취부분음도관세액주진균배양.결과 제일차감염조급제이차감염조혈액급191도관세액중적RAN-TESF인자적함량재감염제7천체도고봉,감염제2천급제14천함량무현저변화,함량균고우대조조.제이차감염조동시기RAN.TESF인자함량교제일차감염조현저증다.제일차감염조급제이차감염조동시기음도관세액중RANTESF인자함량교혈액중현저증다.음도관세액진균배양현시제일차、제이차감염조cfu계수우제7천체도고봉,제14천여제2천무현저차이.제이차감염조동시기진균배양cfu계수비제일차감염조현저증다.결론 RANTESF인자재항백색가사효모균적감염과정중발휘료작용,주요시통과음도국부점막면역발휘작용.차재재차감염과정중발휘교대작용.
Objective To study the kinetic expression level of chemokines (RANTESF) in the murine infection of vulvovaginal can-didiasis (VVC), and explore the function of chemokines in local immunity of VVC. Method Sixty-three female Kunming mice, at 8 ～ 10 weeks of age, were used in this study. All animals were divided into three groups. The content variation of RANTESF in blood and yaginal fluids and CFU of vaginal fluid in each separate group of mice were detected at days 2, 7, and 14 after infection. The first group was control group. The second group was infected only one time and the third group was infected twice. The results were analyzed with SPSS 13.0 statis-tical software. Results The content variation of RANTESF and CFU in vaginal fluid reached highest at days 7 in both the first and the sec-ond groups, as well as in the blood. There were no notable changes at days 2 and 14. The content variation in vaginal fluid or blood of the second group was higher than that in the first group after infection. Conclusion CMI, as a host defense mechanism, plays an important role in protecting against vulvovaginal candidiasis, especially in secondary infection. Local innate immunity is more important than systemic in-nate immunity for protection against vulvovaginal candidiasis. Cytokine about RANTES can promote innate immunity modulation; especially the local innate immunity modulation can promote the ehemotaxis of RANTES.