CAJ | 학술논문

目的探讨miRNAlet7( let7)、Ras在非小细胞肺癌中的表达及临床病理学意义.方法利用原位杂交方法检测let7、免疫组化方法检测Ras在68例非小细胞肺癌(肺癌组)及20例癌旁正常肺组织(对照组)中的表达.结果 let7在肺癌组中的阳性表达率(39.7％)低于对照组(75.0％)(P＜0.05)、Ras在肺癌组中的阳性表达率(66.2％)高于对照组(25.0％)(P＜0.0l)；let7阳性率与患者年龄、性别及肿瘤组织类型、分化程度、分期无关(P＞0.05),Ras阳性率与吸烟史、患者性别及肿瘤组织类型有关(P＜0.01),与肿瘤分化程度、淋巴结转移及临床分期无关(P＞0.05)；let7与Ras在非小细胞肺癌组织中表达呈明显负相关(r=-0.627,P＜0.01)；let7阳性表达组2年生存率明显优于阴性表达组(x2 =4.84,P＜0.05)；Ras阳性表达与阴性表达组2年生存率差异无统计学意义(P＞0.05).结论 let7的低表达、Ras的高表达参与了肺癌的发生发展,let7阳性表达与预后密切相关；Ras可协同参与肺癌的发生发展.
목적 탐토miRNAlet7( let7)、Ras재비소세포폐암중적표체급림상병이학의의.방법 이용원위잡교방법검측let7、면역조화방법검측Ras재68례비소세포폐암(폐암조)급20례암방정상폐조직(대조조)중적표체.결과 let7재폐암조중적양성표체솔(39.7％)저우대조조(75.0％)(P＜0.05)、Ras재폐암조중적양성표체솔(66.2％)고우대조조(25.0％)(P＜0.0l)；let7양성솔여환자년령、성별급종류조직류형、분화정도、분기무관(P＞0.05),Ras양성솔여흡연사、환자성별급종류조직류형유관(P＜0.01),여종류분화정도、림파결전이급림상분기무관(P＞0.05)；let7여Ras재비소세포폐암조직중표체정명현부상관(r=-0.627,P＜0.01)；let7양성표체조2년생존솔명현우우음성표체조(x2 =4.84,P＜0.05)；Ras양성표체여음성표체조2년생존솔차이무통계학의의(P＞0.05).결론 let7적저표체、Ras적고표체삼여료폐암적발생발전,let7양성표체여예후밀절상관；Ras가협동삼여폐암적발생발전.
Objective To study the expressions of miRNAlet7 and Ras in non-small cell lung cancer ( NSCLC),and their correlations with clinicopathological features and survival time.Methods In-situ hybridization was used to detect the expression of let7,and SP immunohistochemistry to measure HMGA2 in 68 NSCLC cases ( group A) and 20 cases with normal lungs ( group B).Results The positive rate of let7 in group A was lower than that in group B (39.7％ vs 63.2％ ) ( P ＜0.05).The positive rate of Ras in group A was higher than that in group B (66.2％ vs 25.0％ ) ( P ＜0.01 ).The positive rate of let7 was not related to the age,gender,histological type,cell differentiation,and clinical stages of cancer patients(P ＞0.05).The positive rate of Ras was related to smoking,sex,and histological type of cancer( P ＜0.01 ),and was not related to cell differentiation,lymphatic metastasis,and clinical stages of cancer ( P ＞0.05).There was an obvious negative correlation between let7 and Ras( r =-0.627,P ＜0.01 ).The 2-year survival rate of let7-positive group was higher than that of the let 7-negative group ( x2 =4.84,P ＜0.05).No statistically significant difference was found between Ras-positive and-negative groups ( P ＞0.05 ).Conclusions The lower level of let7 expression,and high level of Ras expression have much to do with the carcinogenesis of NSCLC.The level of let7-positive expression is closely related to prognosis; while Ras may act in cooperativity in the occurrence and development of NSCLC.