中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2012年
7期
427-430
,共4页
魏路清%李学任%刘斌%彭守春
魏路清%李學任%劉斌%彭守春
위로청%리학임%류빈%팽수춘
特发性肺纤维化%支气管肺泡灌洗液%血清%表面加强激光解吸-电离飞行时间质谱技术%蛋白质组学
特髮性肺纖維化%支氣管肺泡灌洗液%血清%錶麵加彊激光解吸-電離飛行時間質譜技術%蛋白質組學
특발성폐섬유화%지기관폐포관세액%혈청%표면가강격광해흡-전리비행시간질보기술%단백질조학
Idiopathic pulmonary fibrosis%Bronchoalveolar lavage fluid%Serum%Surface enhanced laser desorption/ionization time of flight mass spectrometry%Proteomics
目的 筛查特发性肺纤维化(IPF)患者支气管肺泡灌洗液(BALF)和血清中的差异蛋白,以期发现对诊断有价值的生物标志物.方法 应用表面加强激光解吸-电离飞行时间质谱(SELDI-TOF-MS)技术对34例IPF患者(IPF组)和25例非吸烟健康者(对照组)血清和BALF进行检测获得蛋白质指纹图谱,用生物标志物模式软件、生物标志物导向软件对测得的数据进行处理.结果 BALF中共检测出247个蛋白峰,与对照组比较,IPF组13个蛋白质峰差异有统计学意义,其中7个标志物峰强度显著增加,质荷比分别为2240.57、3459.32、3501.78、4146.50、4516.51、4615.88、5651.26(均P＜0.01);6个标志物峰强度显著下降,质荷比分别为4989.91、5043.68、6968.76、11 687.70、11 782.10、14 733.30(均P＜0.01).血清中共检测出142个蛋白峰,与对照组比较,IPF组9个蛋白质峰差异有统计学意义,且9个标志物峰强度均显著增加,质荷比分别为3382.59、3453.39、4608.28、5825.48、8936.76、9164.27、11 525.30、11 689.40、11 886.00(P＜0.05或P＜0.01).结论 SELDI-TOF-MS技术用于IPF患者BALF和血清蛋白质谱分析可筛选出有意义的差异表达蛋白,为进一步发现对IPF诊断有价值的生物标志物提供了实验基础.
目的 篩查特髮性肺纖維化(IPF)患者支氣管肺泡灌洗液(BALF)和血清中的差異蛋白,以期髮現對診斷有價值的生物標誌物.方法 應用錶麵加彊激光解吸-電離飛行時間質譜(SELDI-TOF-MS)技術對34例IPF患者(IPF組)和25例非吸煙健康者(對照組)血清和BALF進行檢測穫得蛋白質指紋圖譜,用生物標誌物模式軟件、生物標誌物導嚮軟件對測得的數據進行處理.結果 BALF中共檢測齣247箇蛋白峰,與對照組比較,IPF組13箇蛋白質峰差異有統計學意義,其中7箇標誌物峰彊度顯著增加,質荷比分彆為2240.57、3459.32、3501.78、4146.50、4516.51、4615.88、5651.26(均P＜0.01);6箇標誌物峰彊度顯著下降,質荷比分彆為4989.91、5043.68、6968.76、11 687.70、11 782.10、14 733.30(均P＜0.01).血清中共檢測齣142箇蛋白峰,與對照組比較,IPF組9箇蛋白質峰差異有統計學意義,且9箇標誌物峰彊度均顯著增加,質荷比分彆為3382.59、3453.39、4608.28、5825.48、8936.76、9164.27、11 525.30、11 689.40、11 886.00(P＜0.05或P＜0.01).結論 SELDI-TOF-MS技術用于IPF患者BALF和血清蛋白質譜分析可篩選齣有意義的差異錶達蛋白,為進一步髮現對IPF診斷有價值的生物標誌物提供瞭實驗基礎.
목적 사사특발성폐섬유화(IPF)환자지기관폐포관세액(BALF)화혈청중적차이단백,이기발현대진단유개치적생물표지물.방법 응용표면가강격광해흡-전리비행시간질보(SELDI-TOF-MS)기술대34례IPF환자(IPF조)화25례비흡연건강자(대조조)혈청화BALF진행검측획득단백질지문도보,용생물표지물모식연건、생물표지물도향연건대측득적수거진행처리.결과 BALF중공검측출247개단백봉,여대조조비교,IPF조13개단백질봉차이유통계학의의,기중7개표지물봉강도현저증가,질하비분별위2240.57、3459.32、3501.78、4146.50、4516.51、4615.88、5651.26(균P＜0.01);6개표지물봉강도현저하강,질하비분별위4989.91、5043.68、6968.76、11 687.70、11 782.10、14 733.30(균P＜0.01).혈청중공검측출142개단백봉,여대조조비교,IPF조9개단백질봉차이유통계학의의,차9개표지물봉강도균현저증가,질하비분별위3382.59、3453.39、4608.28、5825.48、8936.76、9164.27、11 525.30、11 689.40、11 886.00(P＜0.05혹P＜0.01).결론 SELDI-TOF-MS기술용우IPF환자BALF화혈청단백질보분석가사선출유의의적차이표체단백,위진일보발현대IPF진단유개치적생물표지물제공료실험기출.
Objective To screen potential proteins in bronchoalveolar lavage fluid (BALF) and serum samples obtained from patients with idiopathic pulmonary fibrosis (IPF),for the purpose of discovering candidate biomarkers.Methods BALF and serum samples from 34 patients diagnosed IPF (IPF group) and 25 non-smoker healthy controls (control group) were collected.Surface enhanced laser desorption/ionization time of flight mass spectrometry (SELDI-TOF-MS) was used to obtain protein fingerprints from BALF and serum samples.Biomarker Wizard and Biomarker Pattern were introduced in bioinformatics analysis.Results A total of 247 protein peaks were detected in BALF,and 13 peaks with statistical difference compared with control group.Among 13 detected protein peaks,7 with mass/charge ratio (M/Z) values of 2240.57,3459.32,3501.78,4146.50,4516.51,4615.88,5651.26were statistically up-regulated (all P＜0.01);and 6 peaks with M/Z values of 4989.91,5043.68,6968.76,11 687.70,11 782.10,14 733.30 were significantly down-regulated (all P＜0.01 ).In addition,142 protein peaks were differentially expressed in serum samples,and 9 peaks with statistical differences.Among them,9 peaks with M/Z values of 3382.59,3453.39,4608.28,5825.48,8936.76,9164.27,11 525.30,11 689.40 and 11 886.00 were significantly up-regulated (P＜0.05 or P＜0.01 ),compared with control group.Conclusions The present work demonstrated that SELDI-TOF-MS technology be capable of detecting differentially expressed protein peaks in BALF and serum samples from IPF patients.Further investigations were guaranteed to elucidate their potential diagnostic value in clinical practice.
