中华行为医学与脑科学杂志
中華行為醫學與腦科學雜誌
중화행위의학여뇌과학잡지
CHINESE JOURNAL OF BEHAVIORAL MEDICINE AND BRAIN SCIENCE
2009年
11期
963-965
,共3页
方泽漫%吴彩茹%李海红%黄庆军
方澤漫%吳綵茹%李海紅%黃慶軍
방택만%오채여%리해홍%황경군
生命早期应激%母爱剥夺%成年%免疫功能
生命早期應激%母愛剝奪%成年%免疫功能
생명조기응격%모애박탈%성년%면역공능
Early life stress%Maternal deprivation%Adult%Immunity function
目的 观察生命早期应激对小鼠成年后免疫功能的影响.方法 生命早期应激采用新生鼠母爱剥夺和孤养两种方式,分别在小鼠1,2和3月龄检测外周血白细胞比例、刀豆蛋白A(Con A)和脂多糖(LPS)诱导的脾淋巴细胞转化以及外周血清对正常小鼠类别细胞增殖的影响.结果 生命早期母爱剥夺小鼠成年后外周血中性粒细胞比例由(17.94±1.24)%,(17.33±1.80)%和(21.25±2.82)%分别升高至1,2,3月末的(27.17±3.19)%,(29.75±2.76)%和(28.33±3.12)%;淋巴细胞比例降低.由(79.06±1.18)%,(77.83±3.05)%和(72.46±4.45)%分别降低至1,2,3月末的(69.72±4.97)%,(64.00±3.9)%,(66.74±2.67)%.Con A和LPS诱导的脾淋T和B巴细胞转化增强,外周血清对正常淋巴细胞增殖具有显著的抑制作用.结论 生命早期应激可导致小鼠成年后细胞性免疫功能亢进.
目的 觀察生命早期應激對小鼠成年後免疫功能的影響.方法 生命早期應激採用新生鼠母愛剝奪和孤養兩種方式,分彆在小鼠1,2和3月齡檢測外週血白細胞比例、刀豆蛋白A(Con A)和脂多糖(LPS)誘導的脾淋巴細胞轉化以及外週血清對正常小鼠類彆細胞增殖的影響.結果 生命早期母愛剝奪小鼠成年後外週血中性粒細胞比例由(17.94±1.24)%,(17.33±1.80)%和(21.25±2.82)%分彆升高至1,2,3月末的(27.17±3.19)%,(29.75±2.76)%和(28.33±3.12)%;淋巴細胞比例降低.由(79.06±1.18)%,(77.83±3.05)%和(72.46±4.45)%分彆降低至1,2,3月末的(69.72±4.97)%,(64.00±3.9)%,(66.74±2.67)%.Con A和LPS誘導的脾淋T和B巴細胞轉化增彊,外週血清對正常淋巴細胞增殖具有顯著的抑製作用.結論 生命早期應激可導緻小鼠成年後細胞性免疫功能亢進.
목적 관찰생명조기응격대소서성년후면역공능적영향.방법 생명조기응격채용신생서모애박탈화고양량충방식,분별재소서1,2화3월령검측외주혈백세포비례、도두단백A(Con A)화지다당(LPS)유도적비림파세포전화이급외주혈청대정상소서유별세포증식적영향.결과 생명조기모애박탈소서성년후외주혈중성립세포비례유(17.94±1.24)%,(17.33±1.80)%화(21.25±2.82)%분별승고지1,2,3월말적(27.17±3.19)%,(29.75±2.76)%화(28.33±3.12)%;림파세포비례강저.유(79.06±1.18)%,(77.83±3.05)%화(72.46±4.45)%분별강저지1,2,3월말적(69.72±4.97)%,(64.00±3.9)%,(66.74±2.67)%.Con A화LPS유도적비림T화B파세포전화증강,외주혈청대정상림파세포증식구유현저적억제작용.결론 생명조기응격가도치소서성년후세포성면역공능항진.
Objective To investigate the effect of early life stress on peripheral immunity in adult in mice. Methods The neonatal mice were maternal seperation 2 hours everyday from day 2~(nd) to 21~(st), and breeding alone after day 22~(nd). The differential white blood cell count were conducted with blood smear. Concanavalin (Con A) and lipopolysaccharide( LPS) induced splentic proliferation were measured with MTT method, and the effect of serum from stressed mouse on normal lymphocte proliferation were also tested with MTT. Results Adult mice with early life stress showed that neutrophile granulocytes increased from (17. 94 ±1.24)%,( 17. 33 ± 1.80)% and (21.25 ±2.82)% to (27.17 ±3.19)% ,(29.75 ±2.76)% and (28.33 ±3.12)% at 1,2 and 3 months old. Lymphocytes decreased from (79. 06 ± 1. 18 )%, (77. 83 ± 3. 05 )% and (72. 46 ± 4. 45 )% to (69.72 ±4.97)% ,(64.00 ±3.9)% and (66.74 ±2.67)% at 1,2 and 3 months old,and monocyte increased as well. Both Con A and LPS induced splentic proliferation increased in mice with early life stress at 1,2 and 3 months old. Sera from mice with early life stress decreased the normal lymphocyte proliferation compared with sera from control mice. Conclusion Early life maternal deprivation increase cellular immunity in adulthood in mice.
