CAJ | 학술논문

目的观察和比较小鼠腹腔注射人流感病毒H3N2和禽流感病毒H9N2后外周血干扰素γ(interferon-γ,IFN-γ)、肿瘤坏死因子α(tumor necrosis factor-α,TNF-α)和白细胞介素2(interleukin-2,IL-2)质量浓度的变化,探讨其变化规律.方法采用腹腔注射方式给小鼠注射人流感病毒H3N2、禽流感病毒H9N2或无病毒尿囊液,分离血清,用ELISA法检测注射后第1、2、3、4和5天外周血IFN-γ、TNF-α和IL-2质量浓度.分别比较人流感病毒H3N2和禽流感病毒H9N2注射组与阴性对照组在不同时间的IFN-γ、TNF-α和IL-2质量浓度.用方差分析和LSD-t检验进行统计学分析.结果在某些时间段,病毒注射组外周血IFN-γ和IL-2质量浓度与阴性对照组问的差别有统计学意义,而病毒注射组TNF-α质量浓度与阴性对照组间的差别则无统计学意义.结论小鼠腹腔注射人流感病毒H3N2和禽流感病毒H9N2可促进IFN-γ和IL-2产生,而对TNF-α产生则无显著影响.
목적 관찰화비교소서복강주사인류감병독H3N2화금류감병독H9N2후외주혈간우소γ(interferon-γ,IFN-γ)、종류배사인자α(tumor necrosis factor-α,TNF-α)화백세포개소2(interleukin-2,IL-2)질량농도적변화,탐토기변화규률.방법 채용복강주사방식급소서주사인류감병독H3N2、금류감병독H9N2혹무병독뇨낭액,분리혈청,용ELISA법검측주사후제1、2、3、4화5천외주혈IFN-γ、TNF-α화IL-2질량농도.분별비교인류감병독H3N2화금류감병독H9N2주사조여음성대조조재불동시간적IFN-γ、TNF-α화IL-2질량농도.용방차분석화LSD-t검험진행통계학분석.결과 재모사시간단,병독주사조외주혈IFN-γ화IL-2질량농도여음성대조조문적차별유통계학의의,이병독주사조TNF-α질량농도여음성대조조간적차별칙무통계학의의.결론 소서복강주사인류감병독H3N2화금류감병독H9N2가촉진IFN-γ화IL-2산생,이대TNF-α산생칙무현저영향.
Objective To observe changes of IFN-γ, TNF-α and IL-2 levels in mouse peripheral blood following intraperitoneal injection with influenza viruses. Methods Mice were divided into 3 groups randomly and injected with human H3N2 influenza virus, avian H9N2 influenza virus and sterilized virus-free allantoic fluid, respectively. Sera in different groups were collected at several time points after virus injection, and the levels of IFN-γ, TNF-α and IL-2 in peripheral blood were tested by sandwich ELISA. Statistical analysis was made using analysis of variance and LSD-t test. Results The levels of IFN-γ and IL-2 in peripheral blood in influenza viruses injection groups were significantly higher than those in negative control group, and no significant difference in TNF-α level was found between influenza viruses injection groups and negative control group. Conclusion Intraperitoneal injection with human or avian influenza viruses can promote the production of IFN-γ and IL-2 in mouse peripheral blood, but it has little effect on the production of TNF-α.