中华器官移植杂志
中華器官移植雜誌
중화기관이식잡지
CHINESE JOURNAL OF ORGAN TRANSPLANTATION
2009年
10期
589-593
,共5页
陶章%李惠萍%陈学进%余慧%胡洋%姜寒水
陶章%李惠萍%陳學進%餘慧%鬍洋%薑寒水
도장%리혜평%진학진%여혜%호양%강한수
肺损伤%博来霉素%胚胎干细胞
肺損傷%博來黴素%胚胎榦細胞
폐손상%박래매소%배태간세포
Lung injure%Bleomycin%Embryonic stem cell
目的 观察胚胎干细胞移植对博来霉素所致小鼠肺组织损伤的治疗作用,并探讨其作用机制.方法 将60只C57BL/6J小鼠随机分为6组,每组10只,分别为:空白对照组、不移植对照组、移植1次组、移植2次组、移植3次组和移植4次组;除空白对照组外,其余5组均经小鼠气管将博来霉素注射至肺部,制作肺损伤模型,然后分别将S8胚胎干细胞诱导分化而来的上皮祖细胞经4个移植组小鼠尾静脉注入其体内1～4次,空白对照组和不移植对照组注射等体积的生理盐水.实验28 d后,观察各组小鼠的存活时间;肺组织病理学变化;检测肺组织羟脯氨酸含量及肺组织匀浆中转化生长因子β_1和γ干扰素含量;并用β-半乳糖苷酶原位染色方法检测肺组织中上皮祖细胞定植情况.结果 (1)各组小鼠死亡率分别为:空白对照组无死亡,不移植对照组60%,移植1次组50%、移植2次组40%、移植3次组40%和移植4次组20%.(2)肺组织羟脯氨酸含量和肺组织匀浆中TGF-β_1含量由高到低依次为不移植对照组、移植1次组、移植2次组、移植3次组、移植4次组和空白对照组;肺组织匀浆中IFN-γ含量则相反;组间两两比较,差异均有统计学意义(P<0.05).(3)各组肺组织纤维化程度由重到轻依次为不移植对照组、移植1次组、移植2次组、移植3次组、移植4次组和空白对照组;除不移植对照组和移植1次组之间比较,差异无统计学意义外,其余各组之间两两比较,差异均有统计学意义(P<0.05).(4)随着移植上皮祖细胞次数增加,其在肺内定植的时间也相应延长,各组之间两两比较,差异均有统计学意义(P<0.05).结论 多次移植经S8胚胎干细胞诱导分化而来的上皮祖细胞可减轻博来霉素致小鼠的肺损伤;其作用机制可能与上皮祖细胞对肺组织中转化生长因子β_1和γ-干扰素等细胞因子的影响有关.
目的 觀察胚胎榦細胞移植對博來黴素所緻小鼠肺組織損傷的治療作用,併探討其作用機製.方法 將60隻C57BL/6J小鼠隨機分為6組,每組10隻,分彆為:空白對照組、不移植對照組、移植1次組、移植2次組、移植3次組和移植4次組;除空白對照組外,其餘5組均經小鼠氣管將博來黴素註射至肺部,製作肺損傷模型,然後分彆將S8胚胎榦細胞誘導分化而來的上皮祖細胞經4箇移植組小鼠尾靜脈註入其體內1～4次,空白對照組和不移植對照組註射等體積的生理鹽水.實驗28 d後,觀察各組小鼠的存活時間;肺組織病理學變化;檢測肺組織羥脯氨痠含量及肺組織勻漿中轉化生長因子β_1和γ榦擾素含量;併用β-半乳糖苷酶原位染色方法檢測肺組織中上皮祖細胞定植情況.結果 (1)各組小鼠死亡率分彆為:空白對照組無死亡,不移植對照組60%,移植1次組50%、移植2次組40%、移植3次組40%和移植4次組20%.(2)肺組織羥脯氨痠含量和肺組織勻漿中TGF-β_1含量由高到低依次為不移植對照組、移植1次組、移植2次組、移植3次組、移植4次組和空白對照組;肺組織勻漿中IFN-γ含量則相反;組間兩兩比較,差異均有統計學意義(P<0.05).(3)各組肺組織纖維化程度由重到輕依次為不移植對照組、移植1次組、移植2次組、移植3次組、移植4次組和空白對照組;除不移植對照組和移植1次組之間比較,差異無統計學意義外,其餘各組之間兩兩比較,差異均有統計學意義(P<0.05).(4)隨著移植上皮祖細胞次數增加,其在肺內定植的時間也相應延長,各組之間兩兩比較,差異均有統計學意義(P<0.05).結論 多次移植經S8胚胎榦細胞誘導分化而來的上皮祖細胞可減輕博來黴素緻小鼠的肺損傷;其作用機製可能與上皮祖細胞對肺組織中轉化生長因子β_1和γ-榦擾素等細胞因子的影響有關.
목적 관찰배태간세포이식대박래매소소치소서폐조직손상적치료작용,병탐토기작용궤제.방법 장60지C57BL/6J소서수궤분위6조,매조10지,분별위:공백대조조、불이식대조조、이식1차조、이식2차조、이식3차조화이식4차조;제공백대조조외,기여5조균경소서기관장박래매소주사지폐부,제작폐손상모형,연후분별장S8배태간세포유도분화이래적상피조세포경4개이식조소서미정맥주입기체내1～4차,공백대조조화불이식대조조주사등체적적생리염수.실험28 d후,관찰각조소서적존활시간;폐조직병이학변화;검측폐조직간포안산함량급폐조직균장중전화생장인자β_1화γ간우소함량;병용β-반유당감매원위염색방법검측폐조직중상피조세포정식정황.결과 (1)각조소서사망솔분별위:공백대조조무사망,불이식대조조60%,이식1차조50%、이식2차조40%、이식3차조40%화이식4차조20%.(2)폐조직간포안산함량화폐조직균장중TGF-β_1함량유고도저의차위불이식대조조、이식1차조、이식2차조、이식3차조、이식4차조화공백대조조;폐조직균장중IFN-γ함량칙상반;조간량량비교,차이균유통계학의의(P<0.05).(3)각조폐조직섬유화정도유중도경의차위불이식대조조、이식1차조、이식2차조、이식3차조、이식4차조화공백대조조;제불이식대조조화이식1차조지간비교,차이무통계학의의외,기여각조지간량량비교,차이균유통계학의의(P<0.05).(4)수착이식상피조세포차수증가,기재폐내정식적시간야상응연장,각조지간량량비교,차이균유통계학의의(P<0.05).결론 다차이식경S8배태간세포유도분화이래적상피조세포가감경박래매소치소서적폐손상;기작용궤제가능여상피조세포대폐조직중전화생장인자β_1화γ-간우소등세포인자적영향유관.
Objective To investigate the curative effectiveness and action mechanism of embryonic stem cells(ESCs)transplantation for bleomycin-induced pulmonary injury in mice.Methods Sixty C57BL/6J female mice were divided randomly into six groups:control group,bleomycin model group,transplantation group 1,transplantation group 2,transplantation group 3 and transplantation group 4.Those mice except in control group were administrated bleomycin intratraeheally to induce lung injury model,then epithelial progenitor cells from S8-ESC were transplanted into mice from one time to four times respectively in four treatment groups,and the mice in control group and bleomycin model group were administrated 0.9%sodium chloride respectively.After 28 days,the life-span,hydrocyproline concentration in lung tissue,transforming growth factor β_1(TGF-β_1)and interferon-γ (IFN-γ)concentration in lung tissue homogenate were examined,and the pathologic changes of the lung and the engraftment of the epithelial progenitor cells in the injured lung were observed.Results (1)After 28 days,the death rate was 0%(control group),60%(bleomycin model group),50%(transplantation group 1),40%(transplantation group 2),40%(transplantation group 3)and 20%(transplantation group 4)respectively. (2)With the prolonged time of transplanting epithelial progenitor cells to the mice,hydrocyproline and TGF-β_1 concentrations in lung tissue homogenate in bleomycin model group,transplantation group 1,transplantation group 2,transplantation group 3 andtransplantation group 4 became lower and lower,while those in control group were normal.However,an inverse tendency was found in IFN-γ concentrations.Comparisons between each group had significant difference(P<0.05).(3)With the prolonged time of transplanting epithelial progenitor cells to the mice,the severity of the lung injury in bleomycin model group,transplantation group 1,transplantation group 2,transplantation group 3 and transplantation group 4 became slighter and slighter,while the mice in control group was normal.Average rank's comparisons of the severity of the lung injury between each group had significant difference(P<0.05)except that between bleomycin model groups and transplantation group 1.(4) With the increase of transplanted epithelial progenitor cells to the mice,field planting time in the lung became longer and longer,and there was significant difference between each group(P<0.05).Conclusion Transplantation of epithelial progenitor cells from ESCs may relieve the lung injudy induced by bleomycin,and the action mechanism is associated with cytokines such as TGF-β_1,IFN-γ,etc.
