黑龙江农业科学
黑龍江農業科學
흑룡강농업과학
HEILONGJINAG AGRICULTURAL SCIENCE
2012年
2期
90-92
,共3页
红色素%鉴定%生理生化特性
紅色素%鑒定%生理生化特性
홍색소%감정%생리생화특성
red pigment%identification%physiological and chemical characteristics
从海泥中分离纯化得到1株产红色素细菌,对该菌进行生理生化分析和16SrDNA鉴定。结果表明：该菌为革兰氏阴性,H2S、V.P.、酶触及明胶液化试验呈阳性,可在4～37℃下生长。可利用的碳源有麦芽糖、纤维二糖、甘油、D-葡萄糖、D-果糖、海藻糖、蔗糖及D-甘露醇等,可利用的氮源有蛋白胨、酵母膏和牛肉膏等;16SrDNA鉴定为Serratia marcescens,并建立了H2系统发育树。
從海泥中分離純化得到1株產紅色素細菌,對該菌進行生理生化分析和16SrDNA鑒定。結果錶明：該菌為革蘭氏陰性,H2S、V.P.、酶觸及明膠液化試驗呈暘性,可在4～37℃下生長。可利用的碳源有麥芽糖、纖維二糖、甘油、D-葡萄糖、D-果糖、海藻糖、蔗糖及D-甘露醇等,可利用的氮源有蛋白胨、酵母膏和牛肉膏等;16SrDNA鑒定為Serratia marcescens,併建立瞭H2繫統髮育樹。
종해니중분리순화득도1주산홍색소세균,대해균진행생리생화분석화16SrDNA감정。결과표명：해균위혁란씨음성,H2S、V.P.、매촉급명효액화시험정양성,가재4～37℃하생장。가이용적탄원유맥아당、섬유이당、감유、D-포도당、D-과당、해조당、자당급D-감로순등,가이용적담원유단백동、효모고화우육고등;16SrDNA감정위Serratia marcescens,병건립료H2계통발육수。
A bacterial strain producing red pigments was isolated from marine mud,its biochemical and physiological characteristics,16S rDNA sequence were investigated.The result showed that the strain was Gram negative,could produce H2S,2,3-butanediol,peroxidase,gelatinase,the growth temperature was 4~37℃.It could use maltose,cellobiase,glycerol,D-glucose,D-fructose,trehalose,sucrose,D-mannitol etc.as carbon resource,and could use peptone,yeast extract and beef extract as nitrogen resource.The strain H2 was identified as Serratia marcescens by 16S rDNA,and phylogenetic trees were constructed by neighbor joining.
