CAJ | 학술논문

通过单因素实验研究了蜂胶黄酮的超声波提取工艺，并对蜂胶中黄酮类化合物的清除活性氧自由基的能力进行测定。结果表明蜂胶黄酮的最佳提取工艺为：75％乙醇作为溶剂，料液比为1：10，提取时间为20min，超声波提取2次，温度为60cc，此时黄酮提取含量最高，达到5．046mg／g。蜂胶中黄酮提取液稀释100倍后对DPPH活性氧自由基的清除能力和Vc的清除能力相当，蜂胶黄酮是比较有开发潜力的抗氧化剂。
통과단인소실험연구료봉효황동적초성파제취공예，병대봉효중황동류화합물적청제활성양자유기적능력진행측정。결과표명봉효황동적최가제취공예위：75％을순작위용제，료액비위1：10，제취시간위20min，초성파제취2차，온도위60cc，차시황동제취함량최고，체도5．046mg／g。봉효중황동제취액희석100배후대DPPH활성양자유기적청제능력화Vc적청제능력상당，봉효황동시비교유개발잠력적항양화제。
To study the ultrasonic conditions and moni- tor the content of flavonoids in propolis, the monothetic experimental method was used to determine the best condi- tions for extraction of flavonoids in propolis by using ul- trasonic. The anti-oxidant activity was determined by us- ing DPPH. The optimum extract of total flavonoids was ob- tained under the conditions of 60℃, ratio of propolis to solvent 1：10, ethanol concentration 75% and ultrasonic ex- tracting for 20min, continuous 2 time. With the optimized technology, the content of total flavonoids in the final product was 5.046mg/g. Extraction of propolis flavonoids dilution （multiple 100 diluted solution） has the same an- ti-oxidant activity with the same concentration VC. Propo- lis has a bright future for antioxidant exploitation.