南华大学学报：自然科学版
南華大學學報：自然科學版
남화대학학보：자연과학판
Journal of University of South China(Science and Technology)
2011年
4期
105-108
,共4页
枸骨根%总三萜%分光光度法%熊果酸
枸骨根%總三萜%分光光度法%熊果痠
구골근%총삼첩%분광광도법%웅과산
the root of Ilex cornuta%total triterpenes%spectrophotometry%ursolic acid
本文以采集于湘潭县的枸骨根为材料,选用熊果酸为对照品,5%香草醛—冰乙酸和高氯酸为显色剂,以分光光度法测定枸骨根中总三萜的含量.实验结果表明：在549 nm波长下,标准品在4.4～26.4 mg/L范围内,吸光度与含量呈良好的线性关系,其回归方程为：y=0.0357x-0.0809,R2=0.9984.样品吸光度在5～40 min内相对比较稳定,其三萜含量为3.20%,RSD值为0.31%.该方法操作简便,快速,灵敏度高,重复性好.
本文以採集于湘潭縣的枸骨根為材料,選用熊果痠為對照品,5%香草醛—冰乙痠和高氯痠為顯色劑,以分光光度法測定枸骨根中總三萜的含量.實驗結果錶明：在549 nm波長下,標準品在4.4～26.4 mg/L範圍內,吸光度與含量呈良好的線性關繫,其迴歸方程為：y=0.0357x-0.0809,R2=0.9984.樣品吸光度在5～40 min內相對比較穩定,其三萜含量為3.20%,RSD值為0.31%.該方法操作簡便,快速,靈敏度高,重複性好.
본문이채집우상담현적구골근위재료,선용웅과산위대조품,5%향초철—빙을산화고록산위현색제,이분광광도법측정구골근중총삼첩적함량.실험결과표명：재549 nm파장하,표준품재4.4～26.4 mg/L범위내,흡광도여함량정량호적선성관계,기회귀방정위：y=0.0357x-0.0809,R2=0.9984.양품흡광도재5～40 min내상대비교은정,기삼첩함량위3.20%,RSD치위0.31%.해방법조작간편,쾌속,령민도고,중복성호.
The total triterpenoids in the root of Ilex cornuta was quantified by spectrometry method using ursolic acid as standard and 5% vanillin-glacial acetic acid and perchloric acid as color-developing agents.The results showed that the content of the standard was well correlated to its optical density at 549 nm,the calibration curve was linear（R2=0.9984）in the range of 4.4~ 26.4 mg/L within 5~40 min and the curve was y=0.0357x-0.0809.The above method for the quantification was proved to have the advantages of simple operation,rapidity,high sensitivity and fine repetition.
