CAJ | 학술논문

抗菌肽具有分子量小，活性强，抗菌谱广等特性，为非特异性免疫的关键因子。为研究其抑菌活性，取鲫鱼为研究对象，以60％乙腈和1％三氟乙酸为提取液，从鲫鱼的肝、肾、鳃、皮肤粘液中提取抗菌活性物质，用大肠杆菌和鳗弧菌检测其抑菌活性。结果显示，肝、肾抑菌效果明显，皮肤粘液与鳃次之，其抑菌效果有组织特异性；对该提取物进行热敏性检测，热处理样品抑菌圈直径略有减小，结果显示鲫鱼各组织均含有热不敏感的抗菌肽。
항균태구유분자량소，활성강，항균보엄등특성，위비특이성면역적관건인자。위연구기억균활성，취즉어위연구대상，이60％을정화1％삼불을산위제취액，종즉어적간、신、새、피부점액중제취항균활성물질，용대장간균화만호균검측기억균활성。결과현시，간、신억균효과명현，피부점액여새차지，기억균효과유조직특이성；대해제취물진행열민성검측，열처리양품억균권직경략유감소，결과현시즉어각조직균함유열불민감적항균태。
Antibacterial peptide is a key factor of non-specific immunity, which is characterized by low molecular weight, strong activity and effective against a broad spectrum of microorganisms. Antibacterial peptides were extracted from healthy crucian carp tissues including skin mucus, gills,liver, kidney by 60% cyanomethane and 1% trifluoroacetic acid extraction. The results showed strong inhibition of Escherichia coli and Vibrio anguillarum. And the antimicrobial ability varied in different tissues. When the extracts were boiled for 10 min, the antimicrobial activity decreased a little. It was concluded that there were some antibacterial peptides in different tissues of crucian carp,which had strong antimicrobial activity and tissue specificity.