CAJ | 학술논문

通过体外抑菌实验,采用纸片扩散法对家蝇抗菌肽的抑菌性进行定性研究。试验用菌有金黄色葡萄球菌、大肠杆菌、绿脓杆菌、白色念珠菌和黑曲霉菌。在对家蝇抗菌肽抗菌谱研究的基础上,进一步用肉汤稀释法（试管法）定量测定其最低抑菌浓度（MIC）,并对其稳定性进行研究。结果表明,家蝇抗菌肽对金黄色葡萄球菌、绿脓杆菌和大肠杆菌3种细菌有较好的抑制作用,而对白色念珠菌和黑曲霉没有明显的抑制作用,对金黄色葡萄球菌、绿脓杆菌和大肠杆菌最低抑菌浓度（MIC）分别为0.156 mg/mL、0.312 5 mg/mL和6.25 mg/mL。另外,对其稳定性进行讨论,结果表明,家蝇抗菌肽的稳定性良好。
통과체외억균실험,채용지편확산법대가승항균태적억균성진행정성연구。시험용균유금황색포도구균、대장간균、록농간균、백색념주균화흑곡매균。재대가승항균태항균보연구적기출상,진일보용육탕희석법（시관법）정량측정기최저억균농도（MIC）,병대기은정성진행연구。결과표명,가승항균태대금황색포도구균、록농간균화대장간균3충세균유교호적억제작용,이대백색념주균화흑곡매몰유명현적억제작용,대금황색포도구균、록농간균화대장간균최저억균농도（MIC）분별위0.156 mg/mL、0.312 5 mg/mL화6.25 mg/mL。령외,대기은정성진행토론,결과표명,가승항균태적은정성량호。
The in vitro test-disk diffusion method with the staphyloccocus aureus,escherichia coli,pseudomonas aeruginosa,candida albicans and aspergillus niger was employed to research qualitatively the antimicrobial spectrum of housefly.Further,the minimum inhibitory concentration（MIC）was tested by test tube method and stability study was conducted as well.The results showed that good antimicrobial activity against three bacteria was discovered,of which the MIC were 0.156 mg / mL against staphyloccocus aureus,0.312 5 mg / mL against pseudomonas aeruginosa and 6.25 mg / mL against escherichia coli respectively.In the other hand,almost no inhibitory effect was found on candida albicans and aspergillus niger.And good stability of antimicrobial peptide from housefly was confirmed.