CAJ | 학술논문

目的：研究Hedgehog信号转导通路对人乳腺癌细胞MDA—MB-231c—jun、uPA表达的影响和作用机制，认识其在侵袭转移中的意义。方法：应用Hedgehog信号通路抑制剂Cyclopamine作用人乳腺癌细胞MDA—MB-231，Transwell小室进行侵袭迁移实验；Western blotting检测P—c—itin、uPA蛋白的水平：RT—PCR检测Gli1、c—jun和uPA的mRNA水平。结果：应用Cyclopamine后，细胞的侵袭迁移能力明显下降；P—c—Jun、uPA蛋白表达减少；Gli1、c—iun和uPAmRNA水平降低。结论：Hedgehog信号通路可通过活化c—jun上调uPA的表达，促进乳腺癌细胞MDA—MB-231细胞的侵袭转移。
목적：연구Hedgehog신호전도통로대인유선암세포MDA—MB-231c—jun、uPA표체적영향화작용궤제，인식기재침습전이중적의의。방법：응용Hedgehog신호통로억제제Cyclopamine작용인유선암세포MDA—MB-231，Transwell소실진행침습천이실험；Western blotting검측P—c—itin、uPA단백적수평：RT—PCR검측Gli1、c—jun화uPA적mRNA수평。결과：응용Cyclopamine후，세포적침습천이능력명현하강；P—c—Jun、uPA단백표체감소；Gli1、c—iun화uPAmRNA수평강저。결론：Hedgehog신호통로가통과활화c—jun상조uPA적표체，촉진유선암세포MDA—MB-231세포적침습전이。
Objective： To explore the effect and the possible mechanisms of Hedgehog singaling pathway on expression of c - jun, uPA in MDA - MB - 231 cells. Methods： Invasion and metastasis of MDA- MB- 231 cells were evaluated with transwell chamber.The expression of P- c- jun and uPA were detected by Western blot. The mRNA levels of Gli 1, c - jun and uPA were measured by reverse transcriplion - poly - merage chain reaction （ RT - PCR ）. Results ： When MDA - MB - 231 cells were treated with Cyclopamine, invasion and metastasis were depressed. The expression level of P- c- Jun and uPA were decreased. The mRNA levels of Ghl, c-jun and uPA were also reduced. Conclusion： invasion, metastasis and uPA level can be induced by activeled Hedgehog singnaling nathwav with activation of c - iun.