CAJ | 학술논문

目的研究白细胞介素-12（Interleukin 12,IL-12）对小鼠S180实体瘤的抗肿瘤作用并初步探讨其机制.方法将小鼠左后肢皮下接种S180细胞荷瘤后的BaLB/c小鼠随机分为生理盐水组和IL-12组,分别给予生理盐水和IL-12.接种第28天,眼球取血,流式细胞仪分析外周血T细胞亚群的变化;解剖取瘤称重,计算瘤指数和抑瘤率,组织切片进行形态学观察.结果在接种瘤细胞第14天时,IL-12组、生理盐水组的肿瘤大小分别达到（7.56±0.09）mm、（8.52±0.46）mm,到第27天时,IL-12组的肿瘤大小为（5.12±1.56）mm,明显小于生理盐水组（15.34±3.18）mm（P〈0.01）.IL-12组的抑瘤率为（31.85±7.24）%,IL-12组的小鼠瘤指数为（5.51±1.35）,与生理盐水组（7.67±0.67）相比差异具有显著性（P〈0.01）.IL-12组的小鼠血液中CD3＋T、CD4＋T、CD8＋T细胞均高于生理盐水组.生理盐水组小鼠肿瘤细胞无明显坏死,并可见有丝分裂象,IL-12组的小鼠肿瘤组织有明显坏死,核固缩、细胞碎片多,但在瘤细胞之间很少见到淋巴细胞及巨噬细胞.结论 IL-12具有明显的抗肿瘤作用,初步显示涉及改变细胞亚群和诱导细胞凋亡的机制.
목적연구백세포개소-12（Interleukin 12,IL-12）대소서S180실체류적항종류작용병초보탐토기궤제.방법장소서좌후지피하접충S180세포하류후적BaLB/c소서수궤분위생리염수조화IL-12조,분별급여생리염수화IL-12.접충제28천,안구취혈,류식세포의분석외주혈T세포아군적변화;해부취류칭중,계산류지수화억류솔,조직절편진행형태학관찰.결과재접충류세포제14천시,IL-12조、생리염수조적종류대소분별체도（7.56±0.09）mm、（8.52±0.46）mm,도제27천시,IL-12조적종류대소위（5.12±1.56）mm,명현소우생리염수조（15.34±3.18）mm（P〈0.01）.IL-12조적억류솔위（31.85±7.24）%,IL-12조적소서류지수위（5.51±1.35）,여생리염수조（7.67±0.67）상비차이구유현저성（P〈0.01）.IL-12조적소서혈액중CD3＋T、CD4＋T、CD8＋T세포균고우생리염수조.생리염수조소서종류세포무명현배사,병가견유사분렬상,IL-12조적소서종류조직유명현배사,핵고축、세포쇄편다,단재류세포지간흔소견도림파세포급거서세포.결론 IL-12구유명현적항종류작용,초보현시섭급개변세포아군화유도세포조망적궤제.
Objectives To study the antitumor function and mechanism of IL-12 in treating S180 solid tumors in mice.Methods The tumor-bearing mice which were inoculated with S180 cells（1×107/ml） subcutaneously in the left hind limb were divided into two groups：NS control group and IL-12 treatment group.And NS,IL-12 were respectively given to the mice of two groups.The ocular blood was removed from the mouse of each group on the 28th day to analyze.Then CD3＋T,CD4＋T,CD8＋T and the ratio of CD4/CD8 of peripheral blood was analyzed with FCM,and tumor tissue was dissected completely for weighting and calculating the inhibition rate and index.The regular diet and physical conditions of the remaining mice were observed,and their tumor size was measured.Results On the 14th day,tumor size of NS control group,IL-12 group respectively got to（8.52±0.46） mm,（7.56±0.09） mm.Then it began to reduce in the IL-12 group.On the 27th day,tumor size was（5.12±1.56） mm and significantly less than that of NS control group（15.34±3.18） mm（P0.01）.The tumor inhibition rate of IL-12 group was（31.85±7.24）%,significantly higher than that of NS group（P0.01）.Tumor index of NS control group was 7.67 ± 0.67 and tumor index of IL-12 group was 5.51±1.35,which was significantly lower than that of NS control group（P0.01）.CD3＋T,CD4＋T,CD8＋T cells in mice blood of IL-12 group were all higher than that of NS control group.Tumor cells of mice in NS group had no significant necrosis,and Mitosis was observed.Tumor tissue of mice in IL-12 group had significant necrosis with most karyopyknosis and cell debris,but lymphocytes and macrophages between tumor cells rarely seen. Conclusions IL-12 has apparente effects in anti-tumor immune response.And it demonstrats that IL-12 relates to the mechanism of changing cell subpopulation and leading apotosis.