CAJ | 학술논문

干旱、高盐、低温、高温等非生物逆境严重影响植物的生长发育,研究参与逆境胁迫应答基因具有重要的理论意义和应用价值。从玉米中克隆了一个CIPK蛋白激酶基因,暂时命名为ZmCIPK10。该基因全长2730bp,转录长度2100bp,编码438个氨基酸。顺式元件分析发现在基因启动子区域存在ABRE、HSE、TC-rich repeats等推测的逆境顺势元件。荧光实时定量PCR结果表明ZmCIPK10在干旱、低温、盐胁迫下表达量上调,在ABA、高温胁迫下表达量下调。研究结果初步证实ZmCIPK10基因响应非生物逆境胁迫,为ZmCIPK10参与植物逆境信号途径及其功能研究提供理论依据。
간한、고염、저온、고온등비생물역경엄중영향식물적생장발육,연구삼여역경협박응답기인구유중요적이론의의화응용개치。종옥미중극륭료일개CIPK단백격매기인,잠시명명위ZmCIPK10。해기인전장2730bp,전록장도2100bp,편마438개안기산。순식원건분석발현재기인계동자구역존재ABRE、HSE、TC-rich repeats등추측적역경순세원건。형광실시정량PCR결과표명ZmCIPK10재간한、저온、염협박하표체량상조,재ABA、고온협박하표체량하조。연구결과초보증실ZmCIPK10기인향응비생물역경협박,위ZmCIPK10삼여식물역경신호도경급기공능연구제공이론의거。
Drought, high salinity, low temperature and other abiotic stress affect plant growth and development seriously. It has important theoretical and practical value to research key genes involved in stresses. A CIPK protein kinase gene, named ZmCIPK10 tentatively, was cloned in this study. The gene is 2 730 bp long in the genome, 2 100 bp of transcript, encoding 438 amino acids. Cis element analysis identified ABRE, HSE, TC rich repeats and other stresses core sequence within the promoter region of the ZmCIPK10 gene. Real time PCR analysis indicated that the expression of ZmCIPK10 was up regulated under the dehydration, cold and salt treatment and down regulated under ABA and heat treatment. This study demonstrated maize ZmCIPKK10 gene took part in stresses responses,which would be helpful to further illustrate the function of ZmCIPK10 in maize.