CAJ | 학술논문

提出了一种基于SiO2纳米颗粒免疫凝集反应的压电传感器对霍乱毒素的直接检测，研究发现待测物霍乱毒素会同时被晶振表面固定的霍乱毒素抗体和检测体系中SiO：标记的霍乱毒素抗体识别，引起检测体系中的SiO：在传感界面上特异性凝集，从而引起晶振表面的巨大质量变化和检测介质密度和粘度变化。实验结果表明，该方法修饰的探针能够有效地对霍乱毒素进行检测。
제출료일충기우SiO2납미과립면역응집반응적압전전감기대곽란독소적직접검측，연구발현대측물곽란독소회동시피정진표면고정적곽란독소항체화검측체계중SiO：표기적곽란독소항체식별，인기검측체계중적SiO：재전감계면상특이성응집，종이인기정진표면적거대질량변화화검측개질밀도화점도변화。실험결과표명，해방법수식적탐침능구유효지대곽란독소진행검측。
A simple piezoelectric immunoagglutination assay technique has been developed for direct detection of cholera toxin. It was based on the specific agglutination of cholera toxin antibody-coated silica nanoparticles in the presence of cholera toxin. The antibody modified on the probe surface would combine with antibody-coated nanoparticles in the presence of antigen （cholera toxin） when the surface agglutinationreaction took place, which couples both the mass effect and the viscoelastic effect acting on the probe. The results indicated that the probe signal can be observably multiplied.