CAJ | 학술논문

建立了固相萃取净化超高效液相荧光测定调味品中罗丹明B的方法。样品中的罗丹明B经水提取,Strata-X-CW SPE柱萃取净化,用超高效液相色谱（荧光检测器）进行测定。结果表明：罗丹明B在0.1～1 000.0 ng/mL范围内呈现良好的线性关系,相关系数为0.999 86;在1.0～100.0μg/kg添加水平时,平均回收率为72.0%～96.5%,相对标准偏差为1.9%～5.2%（n=6）;定量检出限为0.1μg/kg。该方法灵敏度高,重复性好,快速,适用于调味品中罗丹明B的检测。
건립료고상췌취정화초고효액상형광측정조미품중라단명B적방법。양품중적라단명B경수제취,Strata-X-CW SPE주췌취정화,용초고효액상색보（형광검측기）진행측정。결과표명：라단명B재0.1～1 000.0 ng/mL범위내정현량호적선성관계,상관계수위0.999 86;재1.0～100.0μg/kg첨가수평시,평균회수솔위72.0%～96.5%,상대표준편차위1.9%～5.2%（n=6）;정량검출한위0.1μg/kg。해방법령민도고,중복성호,쾌속,괄용우조미품중라단명B적검측。
Solid phase extraction followed by ultra performance liquid chromatography（UPLC） with fluorescence detector method was developed for the analysis of Rhodamine B in condiment.The separation of Rhodamine B from samples was extracted by water,and purified by Strata-X-CW SPE column,and determined by UPLC with fluorescence detector（FLD）.The calibration curve of the method was linear over the range of 1.0 to 1 000.0 ng/mL with a correlation coefficient of 0.999 86.The recoveries for Rhodamine B standard spiked in the range of 1.0 to 100.0 μg/kg were between 72.0% and 96.5% with a relative standard deviation（RSD） being between 1.9% and 5.2%（n=6）.The detection limit was 0.1 μg/kg.Owing to advantages of good linearity,low limit of detection,high precision and rapidity,this method is suitable for the determination of Rhodamine B in condiment.