CAJ | 학술논문

脱水素（dehydrins,DHNs）是高等植物胚胎发育晚期产生的一类特异多肽,能够逆转细胞脱水所造成的伤害。为明确脱水素性质与功能的关系,本研究从3种棱型大麦分离到Dhn6基因,生物信息学分析其编码的蛋白质序列长度分别为523个（六棱）、502个（四棱）和486个（二棱）氨基酸残基;氨基酸突变位点分析发现该基因具有整体保守性和突变位点偏倚性。蛋白质性质与二级结构分析表明,DHN6是高度亲水的碱性蛋白质,线性结构和自由卷曲为二级结构的主要组分,K-片段参与α-螺旋结构的形成,由此推测DHN6的兼性α-螺旋结构域在稳定膜结构的水合保护体系过程中发挥着重要的生理功能。构建的21个物种DHN6蛋白质系统发生树,发现禾本科植物遗传距离较近,结合核苷酸序列的物种特异性,本研究认为Dhn6基因可作为物种鉴定依据。
탈수소（dehydrins,DHNs）시고등식물배태발육만기산생적일류특이다태,능구역전세포탈수소조성적상해。위명학탈수소성질여공능적관계,본연구종3충릉형대맥분리도Dhn6기인,생물신식학분석기편마적단백질서렬장도분별위523개（륙릉）、502개（사릉）화486개（이릉）안기산잔기;안기산돌변위점분석발현해기인구유정체보수성화돌변위점편의성。단백질성질여이급결구분석표명,DHN6시고도친수적감성단백질,선성결구화자유권곡위이급결구적주요조분,K-편단삼여α-라선결구적형성,유차추측DHN6적겸성α-라선결구역재은정막결구적수합보호체계과정중발휘착중요적생리공능。구건적21개물충DHN6단백질계통발생수,발현화본과식물유전거리교근,결합핵감산서렬적물충특이성,본연구인위Dhn6기인가작위물충감정의거。
Dehydrins（DHNs）,a special polypeptide generated in late embryogenesis of higher plants,could protect the plants from the damage caused by cell dehydration.In order to learn the relationship between characteristics and functions of dehydrins,we extracted Dhn6 genes from 3 typical rowed barleys,and used bioinformatics analysis to find their encoded proteins were composed of 523（six-rowed barley）,502（four-rowed barley） and 486（two-rowed barley） amino acid residues,respectively.Furthermore,we found there were whole conservative traits and mutant sites specificity in this gene by analysis of amino acid mutations.Analyses of characteristics and secondary structure indicated that DHN6 consisted of highly hydrophilic alkaline proteins primarily,and the secondary structure consisted of linear structure and numerous random curls.Moreover,the amphipathic α-helices formed by K-segments might play important roles in protecting membrane structure.The phylogenetic tree of 21 species in this study agreed with the view that Dhn6 gene could be an efficient foundation for identifying and distinguishing of different species associated with special sequences of nucleotides,and closer genetic distance was discovered in gramineae crops.