遗传
遺傳
유전
HEREDITAS(BEIJING)
2010年
3期
219-228
,共10页
基因组:ChIP-Seq%ChIP-chip
基因組:ChIP-Seq%ChIP-chip
기인조:ChIP-Seq%ChIP-chip
genome%ChIP-Seq%ChIP-chip
染色质免疫沉淀(Chromatin immunoprecipitaion,CHIP)技术是分析细胞内生理状态下DNA结合蛋白与基因组DNA相互作用的技术.ChIP与高密度芯(CHIP-chip)或高通量测序(CHIP-Seq)相结合能产生大量的研究数据,在细胞的基因表达调控网络研究中发挥重要作用.文章主要介绍ChiP、ChIP-chip和ChIP-Seq的技术特点以及发展趋势,重点讨论了ChIP-Seq数据分析方法及相关的应用实例.
染色質免疫沉澱(Chromatin immunoprecipitaion,CHIP)技術是分析細胞內生理狀態下DNA結閤蛋白與基因組DNA相互作用的技術.ChIP與高密度芯(CHIP-chip)或高通量測序(CHIP-Seq)相結閤能產生大量的研究數據,在細胞的基因錶達調控網絡研究中髮揮重要作用.文章主要介紹ChiP、ChIP-chip和ChIP-Seq的技術特點以及髮展趨勢,重點討論瞭ChIP-Seq數據分析方法及相關的應用實例.
염색질면역침정(Chromatin immunoprecipitaion,CHIP)기술시분석세포내생리상태하DNA결합단백여기인조DNA상호작용적기술.ChIP여고밀도심(CHIP-chip)혹고통량측서(CHIP-Seq)상결합능산생대량적연구수거,재세포적기인표체조공망락연구중발휘중요작용.문장주요개소ChiP、ChIP-chip화ChIP-Seq적기술특점이급발전추세,중점토론료ChIP-Seq수거분석방법급상관적응용실례.
Chromatin immunoprecipitation(ChIP)is an effective technique to analyze the interactions of DNA binding proteins with the genome in vivo.ChIP coupled with high density microarray(cMP-chip)or high-throughput sequencing (CHIP-Seq)has generated large amount of data and expected to allow the development of a network describing the cellular transcriptional regulation.Here,we reviewed the CHIP,CMP-chip,and ChIP-Seq techniques as well as their perspectives.Focus is given to data analysis of ChIP-Seq and the applications of ChiP-chip and ChIP-Seq.
