CAJ | 학술논문

从常年堆放的腐质豆秆中分离到1组产纤维素酶菌群MO, 并在50 ℃、pH8.0条件下培养, 90 h时达到最高酶活, 活性为1.361 1 IU.该酶最适反应温度为60 ℃, pH为7.0, 在60 ℃以下和pH3～8范围内具有良好的热稳定性和pH稳定性.在最适反应条件下, 该酶的最高活性可达2.13 IU.通过生长动力学研究了菌群内部不同生长阶段pH、酶活和失重率的相互关系, 并通过非变性电泳对酶谱进行初步研究, 得到7条活性条带, 说明MO中具有多种产酶菌株.
종상년퇴방적부질두간중분리도1조산섬유소매균군MO, 병재50 ℃、pH8.0조건하배양, 90 h시체도최고매활, 활성위1.361 1 IU.해매최괄반응온도위60 ℃, pH위7.0, 재60 ℃이하화pH3～8범위내구유량호적열은정성화pH은정성.재최괄반응조건하, 해매적최고활성가체2.13 IU.통과생장동역학연구료균군내부불동생장계단pH、매활화실중솔적상호관계, 병통과비변성전영대매보진행초보연구, 득도7조활성조대, 설명MO중구유다충산매균주.
A cellulase producing microbial community (MO) was isolated from rotted beanstalk compost. When the community was cultured at pH 8.0 and 50 ℃, maximal CMCase activity (1.361 1 IU) is observed in 90 hours. The optimal conditions for enzymatic reaction are 60 ℃ at pH 7.0. High heat and pH stability is observed at temperature lower than 60 ℃ and pH 3～8. CMCase activity of cellulase can reach 2.13 IU at optimal reaction point. Growth kinetics indicate some relationships among pH, CMCase activity and weight loss rate in different growth phases of microbial community. Through cellulase analysis for zymogram via native-PAGE, seven activity bands are observed. This suggests that more than one strain is responsible for the production of enzyme in MO.