山东农业大学学报（自然科学版）
山東農業大學學報（自然科學版）
산동농업대학학보（자연과학판）
JOURNAL OF SHANDONG AGRICULTURAL UNIVERSITY(NATURAL SCIENCE)
2010年
1期
57-59
,共3页
泰山女儿茶%总黄酮%多糖%含量测定
泰山女兒茶%總黃酮%多糖%含量測定
태산녀인다%총황동%다당%함량측정
Nv'er Tea from Taishan%total flavonoids%polysaccharides%content determination
目的建立泰山女儿茶中总黄酮和多糖的含量测定方法.方法以分光光度法测定总黄酮含量,用苯酚-硫酸法测定多糖含量.结果总黄酮含量测定最佳检测波长510 nm,芦丁质量浓度在8～48 μ·mL~(-1)范围内与吸光度呈良好线性关系(r=0.9986),平均回收率99.03%(RSD=1.72%,n=5);多糖含量测定的检测波长490 nm,葡萄糖质量浓度在5～50 μ·mL~(-1)范围内与吸光度呈良好线性关系(r=0.9964),平均回收率99.26%(RSD=1.57%,n=4).结论本方法简便易行,结果稳定可靠,可用于泰山女儿茶的质量控制.
目的建立泰山女兒茶中總黃酮和多糖的含量測定方法.方法以分光光度法測定總黃酮含量,用苯酚-硫痠法測定多糖含量.結果總黃酮含量測定最佳檢測波長510 nm,蘆丁質量濃度在8～48 μ·mL~(-1)範圍內與吸光度呈良好線性關繫(r=0.9986),平均迴收率99.03%(RSD=1.72%,n=5);多糖含量測定的檢測波長490 nm,葡萄糖質量濃度在5～50 μ·mL~(-1)範圍內與吸光度呈良好線性關繫(r=0.9964),平均迴收率99.26%(RSD=1.57%,n=4).結論本方法簡便易行,結果穩定可靠,可用于泰山女兒茶的質量控製.
목적건립태산녀인다중총황동화다당적함량측정방법.방법이분광광도법측정총황동함량,용분분-류산법측정다당함량.결과총황동함량측정최가검측파장510 nm,호정질량농도재8～48 μ·mL~(-1)범위내여흡광도정량호선성관계(r=0.9986),평균회수솔99.03%(RSD=1.72%,n=5);다당함량측정적검측파장490 nm,포도당질량농도재5～50 μ·mL~(-1)범위내여흡광도정량호선성관계(r=0.9964),평균회수솔99.26%(RSD=1.57%,n=4).결론본방법간편역행,결과은정가고,가용우태산녀인다적질량공제.
Objective to establish the methods for determination of total flavonoids and polysaccharides in Nv'er Tea from Taishan.Methods the content of the total flavonoids was determined by spectrophotometry,and the polysaccharide content was measured with the phenol-sulphuric acid method.Results the optimal detective wavelength was 510 nm for the total flavonoids,and satisfactroy linear relationship with the absorbability was achieved when the mass concentration of rutin was within a range of 8～48 u·mL~(-1)(r=0.9986),the average recovery rate was 99.03%(RSD=1.72%,n=5).The optimal detective wavelength was 490 nm for the polysaccharides,there was good linear relationship between the absorbability and the content of glucose in a range of 5～50 u·mL~(-1) (r=0.9964) with an average recovery of 99.26%(RSD=1.57,n=4).Conclusion this method is sensitive,simple and accurate,and it can be used for the quality control of Nv'er Tea from Taishan.
