CAJ | 학술논문

目的探讨铁饱和乳铁蛋白对体外培养的大鼠成骨细胞增殖和分化的影响.方法用酶消化法分离大鼠颅盖骨成骨细胞进行原代培养;乳铁蛋白螯合Fe~(2+)获得铁饱和乳铁蛋白;将其稀释到不同浓度作用于成骨细胞,四唑盐比色法(MTT)测定细胞增殖;碱性磷酸酶法(ALP)测定细胞碱性磷酸酶活性.结果随着时间的增加,各浓度组均可促进成骨细胞增殖及增强碱性磷酸酶活性.其中100 μg/mL浓度组在72 h时细胞增殖数目最大、碱性磷酸酶活性最高,较对照组有统计学意义(P＜0.01).结论铁饱和乳铁蛋白能促进大鼠成骨细胞的增殖和分化.
목적 탐토철포화유철단백대체외배양적대서성골세포증식화분화적영향.방법 용매소화법분리대서로개골성골세포진행원대배양;유철단백오합Fe~(2+)획득철포화유철단백;장기희석도불동농도작용우성골세포,사서염비색법(MTT)측정세포증식;감성린산매법(ALP)측정세포감성린산매활성.결과 수착시간적증가,각농도조균가촉진성골세포증식급증강감성린산매활성.기중100 μg/mL농도조재72 h시세포증식수목최대、감성린산매활성최고,교대조조유통계학의의(P＜0.01).결론 철포화유철단백능촉진대서성골세포적증식화분화.
Objective To study the effects of iron-saturated bovine lactoferrin on the proliferation and differentiation of osteoblasts in vitro.Methods Enzyme digestion were used to isolate osteoblasts from cranium of Wister. After chelating Fe~(2+), Lactoferrin changed into iron-saturated bovine lactoferrin. Different concentrations of iron-saturated bovine lactoferrin were used to act on osteoblasts. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium test(MTT) was used to determine the proliferation of osteoblasts, and the Alkaline Phospase Tase(ALP) activity was also determined. Results As the time increased, every concentration group showed proliferation of osteoblasts and enhanced ALP activity. The concentration group of 100 μg/mL at 72 h showed the maximum number of cells and highest ALP activity. Conclusion Iron-saturated bovine lactoferrin can promote the proliferation and differentiation of rat osteoblasts.