CAJ | 학술논문

苯丙氨酸解氨酶(Phenylalanine Ammonia-Lyase,PAL;EC 4.3.1.5)是木质素生物合成过程的关键酶和限速酶.应用RACE(Rapid-Amplification of cDNA Ends)方法,获得了白哺鸡竹(Phyllostachys dulcis)、高节竹(Phyllostachys prominens)、龙鳞竹(Phyllostachys pubescens heterocycla)、丽水苦竹(Pleioblastus maculosoides Wen)等4种竹子PAL基因的全长序列,并进行了生物信息学分析.结果表明,PAL基因的开放读码框长度为2 136 bp,共编码712个氨基酸,具有2个外显子和1个内含子.其中高节竹、龙鳞竹、丽水苦竹PAL基因内含子长度为121 bp,白哺鸡竹PLA基因内含子长度为84 bp.推测其氨基酸序列,并分析PAL蛋白单体的三维结构,结果显示PAL蛋白均含有大量的α螺旋和β折叠结构.基于邻接法的进化树对31个物种的PAL基因的氨基酸序列分析表明,竹类植物PAL基因的保守性较高,与禾本科植物玉米、甘蔗的亲缘关系较近,与双子叶植物辣椒等的亲缘关系较远.
분병안산해안매(Phenylalanine Ammonia-Lyase,PAL;EC 4.3.1.5)시목질소생물합성과정적관건매화한속매.응용RACE(Rapid-Amplification of cDNA Ends)방법,획득료백포계죽(Phyllostachys dulcis)、고절죽(Phyllostachys prominens)、룡린죽(Phyllostachys pubescens heterocycla)、려수고죽(Pleioblastus maculosoides Wen)등4충죽자PAL기인적전장서렬,병진행료생물신식학분석.결과표명,PAL기인적개방독마광장도위2 136 bp,공편마712개안기산,구유2개외현자화1개내함자.기중고절죽、룡린죽、려수고죽PAL기인내함자장도위121 bp,백포계죽PLA기인내함자장도위84 bp.추측기안기산서렬,병분석PAL단백단체적삼유결구,결과현시PAL단백균함유대량적α라선화β절첩결구.기우린접법적진화수대31개물충적PAL기인적안기산서렬분석표명,죽류식물PAL기인적보수성교고,여화본과식물옥미、감자적친연관계교근,여쌍자협식물랄초등적친연관계교원.
Phenylalanine Ammonia-Lyase (PAL EC 4.3.1.5) is one of the key enzymes in the process of lignin synthesis.In this paper,PAL gene sequences from four varieties of bamboo:Phyllostachys dulcis,Phyllostachys prominens,Phyllostachys pubescens heterocycla and Pleioblastus maculosoides Wen,were obtained by Rapid-Amplification of cDNA Ends (RACE).The results show that each PAL gene has two exons and an intron and every Open Reading Frame (ORF) is 2 136 bp in length,encoding 712 amino acids.The bamboo PAL amino acid sequences and threedimensional structures were then analyzed and inferred.The results indicate that all of the PAL proteins contain a large number of α-helix and β plated sheet structure.The neighbor-joining phylogenetic tree based on PAL sequences of bamboo and other 27 plants indicates that PAL gene sequence in bamboo is relatively conservative,and bamboo has a very close relationship with that of Zea may and Saccharum officinarum,and a remote relationship with that of Capsicum annuum et al.